Molecular phylogeny of the Greek populations of the genus Ligidium (Isopoda, Oniscidea) using three mtDNA gene segments
Molecular phylogeny of the Greek populations of the genus Ligidium (Isopoda, Oniscidea) using three mtDNA gene segments
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DOI：
10.1111/j.1463-6409.2006.00243.x
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发表时间：
2006-09-01
影响因子：
2.5
通讯作者：
Parmakelis, Aristeidis
中科院分区：
文献类型：
--
作者：
Klossa-Kilia, Elena;Kilias, George;Parmakelis, Aristeidis
基于线粒体12SrRNA、16SrRNA和COI基因片段，对女贞属（Ligidium）希腊居群的遗传进化进行了重建。两个广泛分布的欧洲种，以及三个外类群属于不同的等足类属，也包括在分析中。所使用的样本代表了几乎所有已知在希腊出现的女贞属物种，以及几个未知特定状态的种群和一些新记录。系统发育分析的组合数据集进行贝叶斯推断和最大简约。两个主要的姐妹分支具有良好的支持，表明在希腊大陆南部（伯罗奔尼索斯），其中Ligidium种群表现出镶嵌分布的兄弟种的两个谱系的同域分化。爱琴海群岛的岛屿人口显示出增加的遗传分化，并形成独立的分支。分子遗传学和形态学都强烈地表明存在亚洲起源的第三谱系。唯一可能有效的诊断形态特征表现出只有部分对应支持的分子进化分支。种群之间的遗传分化非常高，这一事实可以归因于这些动物严格的生态专业化，导致即使在非常接近的种群之间也会增加隔离程度。因此，希腊女贞种群，特别是那些存在于岛屿上的种群，是独特的基因库，极容易灭绝。
The phylogeny of Greek populations of the terrestrial isopod genus Ligidium is reconstructed based on three mtDNA gene segments: 12S rRNA, 16S rRNA and COI. Two widely distributed European species, as well as three outgroups belonging to different isopod genera, were also included in the analyses. The samples used represent almost all Ligidium species known to occur in Greece, as well as several populations of unknown specific status plus some new records. Phylogenetic analyses of the combined data set were performed using Bayesian inference and maximum parsimony. The two main sister clades with good support indicate the sympatric differentiation of two lineages in southern continental Greece (Peloponnisos), where Ligidium populations exhibit a mosaic distribution of sibling species. The insular populations of the Aegean Islands show increased genetic divergence and form separate clades. The presence of a third lineage of Asiatic origin is strongly suggested by both the molecular phylogeny and morphology. The only presumably valid diagnostic morphological character exhibits only partial correspondence to well supported clades of the molecular phylogeny. Genetic differentiation between populations is very high, a fact that can be attributed to the strict ecological specialization of these animals that leads to increased levels of isolation even between populations that are in close proximity. As a consequence, Greek Ligidium populations, especially those present on islands, are unique genetic pools and extremely vulnerable to extinction.