The use of RNAi technologies for gene knockdown in zebrafish
The use of RNAi technologies for gene knockdown in zebrafish
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DOI：
10.1093/bfgp/elr014
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发表时间：
2011-07-01
影响因子：
4
通讯作者：
Hurlstone, Adam F.
中科院分区：
文献类型：
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作者：
Kelly, Amanda;Hurlstone, Adam F.
尽管斑马鱼是研究发育和疾病模型的流行和通用的模式生物，但由于缺乏可靠，稳定和具有成本效益的基因敲除方法，斑马鱼的使用受到阻碍。因此，RNAi作为一种可利用的转录后基因调控方法的发现在斑马鱼研究界引起了很大的兴奋，这并不奇怪。然而，尽管在该领域做出了共同努力，但在斑马鱼中使用RNA干扰技术的进展极其缓慢，并且RNA干扰介导的基因敲除的可靠方法仍然难以捉摸。下文审查了迄今为止在这一领域取得的进展，突出了已查明的主要缺陷，并提出了今后可能的方向。
Despite being a popular and versatile model organism in which to study development and model disease, the use of zebrafish has been hampered by the lack of a reliable, stable and cost-effective method of gene knockdown. It is therefore not surprising that the discovery of RNAi as an exploitable method of post-transcriptional gene regulation has created a lot of excitement within the zebrafish research community. However, despite concerted efforts in the field, progress in the use of RNAi technologies in zebrafish has been extremely slow and a reliable means of RNAi-mediated gene knockdown remains elusive. The following reviews progress in the field to date, highlights the major pitfalls identified and suggests possible future directions.