Physiology and antennal lobe projections of olfactory receptor neurons from sexually isomorphic sensilla on male Heliothis virescens
Physiology and antennal lobe projections of olfactory receptor neurons from sexually isomorphic sensilla on male Heliothis virescens
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DOI：
10.1007/s00359-007-0220-3
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发表时间：
2007-06-01
影响因子：
2.1
通讯作者：
Vickers, N. J.
中科院分区：
文献类型：
--
作者：
Hillier, N. K.;Vickers, N. J.
采用切感器记录和钴-赖氨酸染色法研究了烟夜蛾雄性成虫性同形短触角感受器内嗅觉受体神经元的神经生理和触角叶投射。根据嗅觉反应和嗅觉神经元的生理特性，将202个感器分为14类。72%的感器识别含有ORN刺激同种气味。此外，大量的ORN对植物来源的挥发物N-香紫苏烯（N = 41）特别敏感。起源于与个别感器的ORN的轴突用钴赖氨酸（N = 67）染色，并追踪到触角叶中的个别肾小球。对雌性性信息素成分有反应的ORN表现出与先前描述的雄性H.绿色的额叶轴突分支的ORN敏感的hairpencil组件也位于肾小球附近的触角神经的基础，而那些敏感的植物气味投射到更内侧的肾小球。与雌性H. virescens支持这样的观点，即触角神经基部的肾小球与同种和种间的气味物质有关，而位于中部的肾小球与植物挥发物有关。
The neurophysiology and antennal lobe projections of olfactory receptor neurons (ORNs) within sexually isomorphic short trichoid sensilla of male Heliothis virescens (Noctuidae: Lepidoptera) were investigated using cut-sensillum recording and cobalt-lysine staining. A total of 202 sensilla were sorted into 14 possible sensillar categories based on odor responses and physiology of ORNs within. Seventy-two percent of the sensilla identified contained ORNs stimulated by conspecific odors. In addition, a large number of ORNs were specifically sensitive to N-caryophyllene, a plant-derived volatile (N = 41). Axons originating from ORNs associated with individual sensilla were stained with cobalt lysine (N = 67) and traced to individual glomeruli in the antennal lobe. ORNs with responses to female sex pheromone components exhibited similar axonal projections as those previously described from ORNs in long sensilla trichodea in male H. virescens. Antennal lobe axonal arborizations of ORNs sensitive to hairpencil components were also located in glomeruli near the base of the antennal nerve, whilst those sensitive to plant odorants projected to more medial glomeruli. Comparisons with ORNs described from female H. virescens supports the notion that glomeruli at the base of the antennal nerve are associated with conspecific and interspecific odorants, whereas those located medially are associated with plant volatiles.