Tanshinone IIA protects against pulmonary arterial hypertension in broilers
Tanshinone IIA protects against pulmonary arterial hypertension in broilers
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丹参酮 IIA 可预防肉鸡肺动脉高压
DOI：
10.3382/ps/pew322
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发表时间：
2017-05-01
期刊：
影响因子：
4.4
通讯作者：
Liu, Ping
中科院分区：
文献类型：
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作者：
Hu, Guoliang;Song, Yalu;Liu, Ping
本试验旨在研究丹参酮Ⅱ A（TIIA）对肉鸡肺动脉高压（PAH）的影响。选用200只刚孵出的爱拔益加商品代肉仔鸡，随机分为3组。除对照组（自来水）外，所有组均从第15天开始给予NaCl水（0.3%），保护组中的肉鸡从第15天开始饲喂添加TIIA（2.5 g/kg）的饲料。分别于第28、35、42、49天测定各组鸡右心室重量与总心室重量的比值（RV：TV）及其它生化指标。采用酶联免疫吸附试验（ELISA）测定白细胞介素-6（IL-6）、白细胞介素-1 β（IL-1 β）、核因子κ（NF-κ B）和P38（一种促分裂原活化蛋白激酶）的浓度。结果表明，RV：TV比值在0.250 ~ 0.299范围内（10%）的病鸡比例（25 ~ 30%）显著高于其他各组（P < 0.05），占全部病鸡的28%。此外，IL-6、IL-1 β、NF-κ B B和P38蛋白浓度在患病组中较高，而对照组和保护组之间没有差异。此外，患病组的体重、肝功能、肾功能和电解质的测量结果与其他组之间存在显著差异。这些发现表明丹参酮IIA可以保护肉鸡免受PAH的侵害，这对家禽业来说是一个重要的信息。
This investigation was conducted to study the effects of tanshinone IIA (TIIA) on pulmonary arterial hypertension (PAH) in broilers. Two hundred newly hatched Arbor Acre commercial broilers were randomly divided into 3 groups. All groups, with the exception of the control group (tap water), were given NaCl water (0.3%) starting on the d 15, and broilers in the protected group were fed a diet supplemented with TIIA (2.5 g/kg) starting on the d 15. On d 28, 35, 42, and 49, the ratio of the right ventricular weight to the total ventricular weight (RV: TV) and the values of other biochemical indicators for each group chickens were determined. The concentrations of interleukin-6 (IL-6), interleukin-1 beta (IL-1 beta), nuclear factor kappa (NF-kappa B), and P38 (a mitogen-activated protein kinase) were measured using enzyme-linked immune sorbent assays (ELISA). The results showed that the proportion of chickens in the diseased group with an RV:TV ratio in the range of 0.250 to 0.299 (10%) was significantly higher (25 to 30%) compared to that of the other groups (P < 0.05), and the proportion in all chickens was 28%. In addition, the IL-6, IL-1 beta, NF-kappa B, and P38 protein concentrations were higher in the diseased group, whereas there were no differences between the control group and the protected group. Moreover, the measurements of body weight, liver function, kidney function and electrolytes showed significant differences between the diseased group and the other groups. These findings suggest that tanshinone IIA may protect broilers from PAH, which is an important piece of information for the poultry industry.