Screening of isolated lactic acid bacteria as potential beneficial strains for fermented liquid pig feed production
Screening of isolated lactic acid bacteria as potential beneficial strains for fermented liquid pig feed production
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DOI：
10.1016/j.anifeedsci.2008.08.002
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发表时间：
2009-03-30
影响因子：
3.2
通讯作者：
Heyndrickx, M.
中科院分区：
文献类型：
--
作者：
Missotten, J. A. M.;Goris, J.;Heyndrickx, M.
介绍了生产猪发酵液体饲料(FLF)的潜在益生菌菌株的筛选。从本实验室初步试验的仔猪回肠、屠宰猪盲肠和断奶仔猪盲肠中分离到乳酸菌146株。用重复元件聚合酶链式反应和部分16S rRNA测序对菌株进行鉴定，并对其酸化能力进行筛选。筛选出10株菌株，检测其培养上清液对沙门氏菌的抗菌活性。此外，对它们在30℃下分批发酵72h，在醋酸浓度小于40 mmoL/L，乳酸浓度大于100mmoL/L，pH<4.5的情况下进行了体外分批发酵试验。结果表明，3株乳杆菌MB 3123(约翰逊乳杆菌)、MB 3182(唾液乳杆菌群)和MB 3083(L植物乳杆菌)对FLF的产生有很强的促进作用。加入该菌株后，发酵24 h后FLF的pH值从5.9降至4.2、4.3和4.4，发酵48 h后降至4.1。此外，这三株菌对沙门氏菌也表现出较高的抗菌活性。发酵48h，乳酸产量可达100mmoL/L以上。MB3170菌株在48h后乳酸浓度提高到100mmoL/L以上，而在24h时pH值仅为4.5。结果表明，筛选出能够发酵饲料并在猪的胃肠道中产生大量可能具有抑菌作用的乳酸的益生菌是可能的。(C)2008爱思唯尔B.V.保留所有权利。
In this paper the selection of potential probiotic strains for producing fermented liquid feed (FLF) for pigs is presented. One hundred and forty six strains of lactic acid bacteria were isolated from ileal digesta of piglets, cecal digesta of slaughter pigs and FLF from a preliminary trial at our laboratory. The strains were identified with repetitive-element PCR and partial 16S rRNA sequencing and screened for acidification potential. Ten of the strains were selected and their culture supernatant was checked for antibacterial activity against Salmonella spp. Also, they were tested in vitro in batch fermentations at 30 degrees C for 72 h, for their capacity to produce FLF (water:feed ratio of 4:1) with a concentration of acetic acid less than 40 mmol/L and of lactic acid above 100 mmol/L, resulting in a pH below 4.5. The Lactobacillus strains MB 3123 (L. johnsonii), MB 3182 (L. salivarius group) and MB 3083 (L plantarum) were found to be very effective for the production of FLF The FLF produced by including this strain had a pH drop from 5.9 to 4.2, 4.3 and 4.4 after 24 h and to 4.1 after 48 h of fermentation. Also, these three strains showed high antimicrobial activity against Salmonella spp. and produced more than 100 mmol/L lactic acid after 48 h fermentation. The MB 3170 strain increased the lactic acid concentration above 100 mmol/L after 48 h, but at 24 h the pH was only 4.5. The results indicate that it is possible to screen for probiotic strains capable of fermenting feeds and of producing high quantities of lactic acid with possible antimicrobial effects in the gastro-intestinal tract of pigs. (C) 2008 Elsevier B.V. All rights reserved.