Red wine consumption mitigates the cognitive impairments in low-density lipoprotein receptor knockout (LDLr-/-) mice
Red wine consumption mitigates the cognitive impairments in low-density lipoprotein receptor knockout (LDLr-/-) mice
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DOI：
10.1080/1028415x.2019.1704472
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发表时间：
2020-01-07
影响因子：
3.6
通讯作者：
de Bem, Andreza Fabro
中科院分区：
文献类型：
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作者：
De Paula, Gabriela Cristina;de Oliveira, Jade;de Bem, Andreza Fabro
虽然适量饮用红酒在降低高胆固醇血症相关心血管疾病发病率方面的益处已得到公认，但对于其对高胆固醇血症相关认知障碍的影响仍存在广泛的误解。在这里，我们研究了经常饮用红酒对低密度脂蛋白受体敲除（LDLr-/-）小鼠认知能力的益处，这是一种家族性高胆固醇血症的动物模型，从幼年开始就表现出认知障碍。红酒被稀释到最终浓度为6%乙醇的饮用水中，供喂食正常或高胆固醇饮食的LDLr-/-小鼠使用60天。结果表明，适量饮用红酒不会改变运动参数和肝毒性。在评估空间学习/参考记忆和识别/识别记忆的多项认知任务中，饮用红葡萄酒的高胆固醇血症小鼠的表现明显优于水组，无论饮食如何。此外，免疫荧光分析显示，饮用红酒后，LDLr-/-小鼠海马星形胶质细胞活化和凝集素染色减少。这些发现表明，适量饮用红酒可以减轻小鼠因高胆固醇血症引起的短期和长期记忆衰退，并表明这可能是通过神经血管作用实现的。
Although the benefits of moderate intake of red wine in decreasing incidence of cardiovascular diseases associated to hypercholesterolemia are well recognized, there are still widespread misconceptions about its effects on the hypercholesterolemia-related cognitive impairments. Herein we investigated the putative benefits of regular red wine consumption on cognitive performance of low-density lipoprotein receptor knockout (LDLr-/-) mice, an animal model of familial hypercholesterolemia, which display cognitive impairments since early ages. The red wine was diluted into the drinking water to a final concentration of 6% ethanol and was available for 60 days for LDLr-/- mice fed a normal or high-cholesterol diet. The results indicated that moderate red wine consumption did not alter locomotor parameters and liver toxicity. Across multiple cognitive tasks evaluating spatial learning/reference memory and recognition/identification memory, hypercholesterolemic mice drinking red wine performed significantly better than water group, regardless of diet. Additionally, immunofluorescence assays indicated a reduction of astrocyte activation and lectin stain in the hippocampus of LDLr-/- mice under consumption of red wine. These findings demonstrate that the moderate consumption of red wine attenuates short- and long-term memory decline associated with hypercholesterolemia in mice and suggest that it could be through a neurovascular action.