Nanosecond pulsed electric field thresholds for nanopore formation in neural cells
Nanosecond pulsed electric field thresholds for nanopore formation in neural cells
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DOI：
10.1117/1.jbo.18.3.035005
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发表时间：
2013-03-01
影响因子：
3.5
通讯作者：
Ibey, Bennett L.
中科院分区：
文献类型：
--
作者：
Roth, Caleb C.;Tolstykh, Gleb P.;Ibey, Bennett L.
The persistent influx of ions through nanopores created upon cellular exposure to nanosecond pulse electric fields (nsPEF) could be used to modulate neuronal function. One ion, calcium (Ca2+), is important to action potential firing and regulates many ion channels. However, uncontrolled hyper-excitability of neurons leads to Ca2+ overload and neurodegeneration. Thus, to prevent unintended consequences of nsPEF-induced neural stimulation, knowledge of optimum exposure parameters is required. We determined the relationship between nsPEF exposure parameters (pulse width and amplitude) and nanopore formation in two cell types: rodent neuroblastoma (NG108) and mouse primary hippocampal neurons (PHN). We identified thresholds for nanoporation using Annexin V and FM1-43, to detect changes in membrane asymmetry, and through Ca2+ influx using Calcium Green. The ED50 for a single 600 ns pulse, necessary to cause uptake of extracellular Ca2+, was 1.76 kV/cm for NG108 and 0.84 kV/cm for PHN. At 16.2 kV/cm, the ED50 for pulse width was 95 ns for both cell lines. Cadmium, a nonspecific Ca2+ channel blocker, failed to prevent Ca2+ uptake suggesting that observed influx is likely due to nanoporation. These data demonstrate that moderate amplitude single nsPEF exposures result in rapid Ca2+ influx that may be capable of controllably modulating neurological function. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
