CLONING AND EXPRESSION OF A PROTEIN-KINASE C-REGULATED CHLORIDE CHANNEL ABUNDANTLY EXPRESSED IN RAT-BRAIN NEURONAL CELLS
CLONING AND EXPRESSION OF A PROTEIN-KINASE C-REGULATED CHLORIDE CHANNEL ABUNDANTLY EXPRESSED IN RAT-BRAIN NEURONAL CELLS
复制标题
DOI：
10.1016/0896-6273(94)90215-1
复制
发表时间：
1994-03-01
期刊：
影响因子：
16.2
通讯作者：
SASAKI, S
中科院分区：
文献类型：
--
作者：
KAWASAKI, M;UCHIDA, S;SASAKI, S
克隆并表征了编码蛋白激酶 C 调节的氯离子通道的 cDNA (CIC-3)。该开放阅读框编码760个氨基酸，与之前克隆的CIC氯化物通道具有显着的氨基酸一致性。注射了 CIC-3 cRNA 的非洲爪蟾卵母细胞中表达的氯离子电流被 12-O-十四烷酰佛波醇 13-乙酸酯激活蛋白激酶 C 完全阻断。在大鼠脑中观察到CIC-3 mRNA的大量表达，特别是在嗅球、海马和小脑中。这些发现表明，CIC-3 可能通过蛋白激酶 C 调节膜兴奋性在神经细胞功能中发挥重要作用。
cDNA (CIC-3) encoding a protein kinase C-regulated chloride channel was cloned and characterized. The open reading frame encodes 760 amino acids, which possess significant amino acid identity with previously cloned CIC chloride channels. The chloride currents expressed in Xenopus oocytes injected with CIC-3 cRNA were completely blocked by activation of protein kinase C by 12-O-tetradecanoylphorbol 13-acetate. Abundant expression of CIC-3, mRNA was observed in rat brain, especially in the olfactory bulb, hippocampus, and cerebellum. These findings suggest that CIC-3 may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C.