The immobilization of basic fibroblast growth factor on plasma-treated poly(lactide-co-glycolide)
The immobilization of basic fibroblast growth factor on plasma-treated poly(lactide-co-glycolide)
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DOI：
10.1016/j.biomaterials.2008.02.008
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发表时间：
2008-05-01
期刊：
影响因子：
14
通讯作者：
Wang, Shenguo
中科院分区：
文献类型：
--
作者：
Shen, Hong;Hu, Xixue;Wang, Shenguo
本研究探讨了碱性成纤维细胞生长因子（bFGF）在聚内酯型聚合物支架上经等离子体处理固定的可能性。为了在聚合物基体表面引入酸性羧基，用二氧化碳（CO2）等离子体处理聚乳酸-共聚物（PLGA）膜，然后在bFGF的磷酸盐缓冲盐水（PBS, pH 7.4）溶液中孵育。研究并比较了不同处理参数下bFGF与CO2等离子体处理PLGA （PT-PLGA）薄膜的结合效率。结果表明，CO2等离子体处理可提高bFGF与PLGA的结合效率。bFGF与PLGA的结合效率随CO2等离子体处理时间的变化而变化，在功率为20 W的条件下，处理时间为20 min后达到最大值。CO2等离子体处理引起的表面化学和表面形貌的变化是提高结合效率的主要原因。结合的bFGF从膜中连续释放体外达7天。在平行板实验室内进一步测试了CO2等离子体固定在PLGA膜上的bFGF的稳定性。将小鼠3T3成纤维细胞培养在bFGF结合的PLGA （PT-PLGA/bFGF）膜上，经预先血浆处理（20 W, 20 min），结果表明，PT-PLGA/bFGF膜释放的bFGF具有生物活性。固定化bFGF后，细胞在PLGA支架上的粘附和生长均有明显改善。因此，CO2等离子体处理结合bFGF固支的方法不仅可用于输送bFGF，而且可广泛应用于组织工程中支架的表面修饰。(c) 2008 Elsevier Ltd.版权所有。
In this study, possibility of the method of immobilization of basic fibroblast growth factor (bFGF) on polylactone-type polymer scaffolds via plasma treatment was investigated. To introduce acid carboxylic functional groups on the surface of the polymer matrix, poly(lactide-co-glycolide) (PLGA) film was treated with carbon dioxide (CO2) plasma and then incubated in a phosphate buffer saline (PBS, pH 7.4) solution of bFGF. The bFGF binding efficiency to the CO2 plasma-treated PLGA (PT-PLGA) films under different treating parameters was investigated and compared. It was found bFGF binding efficiency to PLGA was enhanced by CO2 plasma treatment. The binding efficiency of bFGF to PLGA was variational with CO2 plasma treating time and it reached a maximum after a treating time of 20 min under the power of 20 W. The changes of surface chemistry and surface topography induced by CO2 plasma treatment played main roles in improving binding efficiency. Bound bFGF was released continuously from the films for up to 7 days in vitro. The stability of bFGF immobilized on PLGA film via CO2 plasma treatment was tested further under dynamic conditions by a Parallel Plate How Chamber. Mouse 3T3 fibroblasts were cultured on the bFGF bound PLGA with a prior plasma treatment (20 W, 20 min) (PT-PLGA/bFGF) film, which showed that bFGF released from PT-PLGA/bFGF film was bioactive. Adhesion and growth of cells on PLGA scaffolds were greatly improved by immobilization of bFGF on them. Therefore, the method Of CO2 plasma treatment combining bFGF anchorage not only was usable in delivering bFGF, but also could be applied extensively for surface modification of scaffolds in tissue engineering. (c) 2008 Elsevier Ltd. All rights reserved.