Experimental Escherichia coli respiratory infection in broilers
Experimental Escherichia coli respiratory infection in broilers
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DOI：
10.2307/1593047
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发表时间：
2000-10-01
期刊：
影响因子：
1.4
通讯作者：
Gyles, CL
中科院分区：
文献类型：
--
作者：
Peighambari, SM;Julian, RJ;Gyles, CL
本研究通过对2 ~ 4周龄肉仔鸡进行禽致病性大肠杆菌气溶胶给药，确定了大肠杆菌病实验繁殖的最佳条件。复制疾病的基本模型是鼻内给予约10（4）平均胚胎感染剂量的传染性支气管炎病毒（IBV），然后气雾剂给予O 2或O 78株E。Horsfall单位的大肠杆菌（100 ml 10（9）菌落形成单位/ml的悬浮液，40 min）。根据死亡情况、气囊、肝脏和心脏病变的频率和严重程度以及攻毒E的恢复情况，对感染鸡组进行评分。E后6天滚动。大肠杆菌感染。IBV与E.无论鸡在8日龄还是20日龄接受IBV，一般情况下，50%~ 80%的鸡发生气囊炎，0%~ 29%的鸡发生心包炎或肝周炎，死亡率很低或无死亡率。大肠杆菌气溶胶通过锥形室给药。单独给予IBV未能诱导病变。挑战E的恢复。鸡的大肠杆菌感染与病变相关性不好。在此基础上，对20日龄鸡进行IBV感染，4天后暴露于禽致病性E.大肠杆菌复制禽大肠杆菌病，具有低死亡率、高气囊炎百分比和低败血性病变百分比，这些是在自然疾病中所见的特征。
This study determined optimal conditions for experimental reproduction of colibacillosis by aerosol administration of avian pathogenic Escherichia coli to 2-to-4-wk-old broiler chickens. The basic model for reproducing disease was intranasal administration of approximately 10(4) mean embryo infectious dose of infectious bronchitis virus (IBV) followed by aerosol administration of an O2 or an O78 strain of E. coli in a Horsfall unit (100 mi of a suspension of 10(9) colony-forming units/ml over 40 min). Scores were assigned to groups of infected chickens on the basis of deaths; frequency and severity of lesions in the air sacs, liver and heart; and recovery of the challenge E. roll 6 days post-E. coli infection. An interval of 4 days between the IBV and E. coli challenges was best whether the chickens received the IBV at 8 or 20 days of age. Typically 50%-80% of the chickens developed airsacculitis and 0 to 29% of the chickens developed pericarditis or perihepatitis, with little or no mortality Escherichia coli alone resulted in no deaths and 0 to 20% airsacculitis, but these percentages increased to 0 to 5% and 52%-60% when the E. coli aerosol was administered through a cone-shaped chamber. Administration of IBV alone failed to induce lesions. Recovery of the challenge E. coli from chickens did not correlate well with lesions. On thr basis of these data, administration of IBV to 20-day-old chickens followed 4 days later by exposure to an avian pathogenic E. coli reproduces avian colibacillosis with the low mortality high percentage of airsacculitis, and low percentage of septicemic lesions characteristic of the conditions seen in the natural disease.