The prediction of T- and B-combined epitope and tertiary structure of the Eg95 antigen of Echinococcus granulosus.
The prediction of T- and B-combined epitope and tertiary structure of the Eg95 antigen of Echinococcus granulosus.
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细粒棘球绦虫Eg95抗原T、B结合表位及三级结构的预测
DOI：
10.3892/etm.2013.1187
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发表时间：
2013-09
影响因子：
2.7
通讯作者：
Ding J
中科院分区：
文献类型：
--
作者：
Ma X;Zhou X;Zhu Y;Li Y;Wang H;Mamuti W;Li Y;Wen H;Ding J
包虫病又称包虫病，是由包虫病幼虫感染引起的一种人畜共患寄生虫病。这种疾病对人和动物都有严重的危害。研究和开发表位疫苗至关重要。为了确定细粒棘球绦虫Eg95抗原的优势表位，本研究对细粒棘球绦虫Eg95蛋白的三级结构和T、B结合表位进行了预测和分析。用3DLigandSite服务器和RasMol软件预测了Eg95蛋白的三级结构。用DNAStar(V5.0)、IEDB、SYFPEITHI和BIMAS分析了Eg95抗原的T-和B-联合表位。三级结构预测结果表明，Eg95抗原中存在潜在的表位。生物信息学分析揭示了Eg95抗原的T-和B-结合表位。四个和六个T-和B-组合表位在人类和小鼠中诱导免疫反应。此外，四个T-和B-组合表位在人类和小鼠中都能诱导免疫反应。此外，还测定了Eg95蛋白的三级结构和T、B结合表位。本研究的结果可能对研究Eg95的抗原性和开发优势表位疫苗具有一定的参考价值。
Echinococcosis, also known as hydatid disease, is a type of zoonotic parasitic disease caused by the Echinococcus larvae infection. The disease is severely harmful to both humans and animals. Research and development of an epitope vaccine is crucial. To determine the dominant epitopes of the Eg95 antigen, the tertiary structure and the T- and B-combined epitope of the Eg95 protein for Echinococcus granulosus were predicted and analyzed in the present study. The tertiary structure of the Eg95 protein was predicted using the 3DLigandsite server and RasMol software. The T- and B-combined epitope of the Eg95 antigen was analyzed using the DNAStar (V5.0), IEDB, SYFPEITHI and BIMAS. Tertiary structure prediction results showed that there were potential epitopes in Eg95 antigen. Bioinformatics analysis revealed the T- and B-combined epitopes of Eg95 antigen. Four and six T- and B-combined epitopes induced immune responses in humans and mice. Additionally, four T- and B-combined epitopes induced immune responses in both humans and mice. The tertiary structure and T- and B-combined epitopes of the Eg95 protein were also determined. The results obtained in the present study may be beneficial in the investigation of Eg95 antigenicity and the development of dominant epitope vaccines.
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影响因子：
2.2
作者：
Lightowlers, MW;Lawrence, SB;Heath, DD
通讯作者：
Heath, DD
影响因子：
4.3
作者：
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影响因子：
3.7
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影响因子：
2.9
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影响因子：
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通讯作者：
Patterson S