Continuous Cultivation of Epithelial Cell Strain (FL) from Human Amniotic Membrane.∗
Continuous Cultivation of Epithelial Cell Strain (FL) from Human Amniotic Membrane.∗
复制标题
人羊膜上皮细胞株 (FL) 的连续培养。*
DOI：
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发表时间：
1957
期刊：
影响因子：
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通讯作者：
R. O. Lund
中科院分区：
文献类型：
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作者：
J. Fogh;R. O. Lund
概述来源于正常羊膜的人细胞株在30次转移中连续传代培养8个月。该细胞系通过胰蛋白酶消化主要来源并在不含胚胎提取物的培养基中培养而形成。在培养的所有阶段，细胞的外观都是上皮样的。报告了在含有人血清或动物血清的培养基中的生长特性。该细胞株已大规模生长。
Summary A strain of human cells derived from a normal amniotic membrane has been cultivated in serial passage for 8 months in 30 transfers. This cell line was developed by trypsinization of the primary source and cultivated in a medium without embryo extract. The appearance of the cell has been epithelial-like at all stages of cultivation. Growth characteristics in media containing human serum or animal sera are reported. The cell strain has been grown in large scale.