Significant different volatile biomarker during bronchoscopic ion mobility spectrometry investigation of patients suffering lung carcinoma
Significant different volatile biomarker during bronchoscopic ion mobility spectrometry investigation of patients suffering lung carcinoma
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DOI：
10.1007/s12127-011-0078-5
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发表时间：
2011-12-01
影响因子：
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通讯作者：
Darwiche, K.
中科院分区：
文献类型：
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作者：
Baumbach, J. I.;Maddula, S.;Darwiche, K.
非小支气管癌患者呼出的气体中含有与健康人不同的挥发性有机化合物（VOC）。即使直接在空气中，也可以使用离子淌度光谱法检测低至 pg/L 范围的 VOC。迄今为止，所发现的不同挥发性有机化合物的来源尚不明确。此类挥发性有机化合物可能是肿瘤代谢的直接产物，或者与大多数存在的辅助因素有关，例如感染或坏死或人体有机体对肿瘤的反应（例如氧化应激）。在本研究中，对 19 名患有不同组织学类型的确诊 NSCLC（非小细胞肺癌）患者的呼吸进行了调查。在所有情况下都实现了灵活的视频芯片支气管镜检查。在对两个主支气管的肺部进行组织学研究之前，使用聚四氟乙烯（PTFE 或特氟龙）管作为导管，直接从支气管镜的工作通道采集空气样本，并将其直接连接到离子淌度谱仪的入口。立即开始测量。总共可以识别 72 个共同峰。 5 肿瘤部位和侧支肺之间的峰值存在显着差异。考虑到腺癌，一个峰将两个位点清楚地分开，并且与正十二烷的二聚体相关。在鳞状细胞癌上发现了两个与 2-丁醇或 2 甲基呋喃和壬醛相关的峰。对于腺癌，2-丁醇和壬醛的敏感性、特异性、阳性和阴性预测值分别为 100%、75%、80% 和 100%；对于鳞状细胞癌，分别为 78%/78%、67%/78%、70%/80% 和 75%/88%。因此，可以使用离子淌度光谱法检测从支气管镜呼吸采样中获得的挥发性有机化合物。本研究表明，不同组织学的肺癌将由不同的挥发性分析物代表。
Exhaled breath of patients suffering non-small bronchial carcinoma contains volatile organic compounds (VOC) different from healthy people. VOCs could be detected using ion mobility spectrometry down to the pg/L range even in air directly. To date, the origin of the different VOCs found is insecure. Such VOCs could be a direct product of the metabolism of the tumor or relatable to mostly present co-factors like infections or necrosis or a reaction of the human organism to the tumor (e. g. oxidativ stress). In the present study the breath of 19 patients suffering from confirmed NSCLC (non-small-cell lung carcinoma) with different histological types was investigated. In all cases flexible video-chip bronchoscopy was realized. Before taking samples for histological investigations in the lung on both main bronchi, samples of air were taken using a polytetrafluoroethylene (PTFE or Teflon) tube as catheter directly from the working channel of a bronchoscope and connected directly to the inlet of the ion mobility spectrometer. The measurement was started immediately. In total, 72 common peaks could be identified. 5 Peaks were significantly varying between the tumor site and the collateral lung. Considering adenocarcinoma, one peak separates both sites clearly and was relatable to the dimer of n-Dodecane. Two peaks were found on squamous cell carcinoma and relatable to 2-Butanol or 2Methylfuran and Nonanal. The sensitivity, specificity, positive and negative predictive values were, for adenocarcinoma 100%, 75%, 80% and 100%, respectively-for squamous cell carcinoma 78%/78%, 67%/78%, 70%/80% and 75%/88%, for 2-Butanol and Nonanal respectively. Therefore, VOCs obtained from bronchoscopic sampling of breath could be detected using ion mobility spectrometry. The present study suggests that lung carcinoma with different histology will be represented by different volatile analytes.