Predicted and observed alleles of Plasmodium falciparum merozoite surface protein-1 (MSP-1), a potential malaria vaccine antigen
Predicted and observed alleles of Plasmodium falciparum merozoite surface protein-1 (MSP-1), a potential malaria vaccine antigen
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DOI：
10.1016/s0166-6851(98)00010-3
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发表时间：
1998-05-01
影响因子：
1.5
通讯作者：
Lal, AA
中科院分区：
文献类型：
--
作者：
Qari, SH;Shi, YP;Lal, AA
恶性疟原虫裂殖子表面蛋白（MSP）-1的19-kDa抗原结构域是一个潜在的疟疾疫苗候选者。基于氨基酸替换，已经鉴定了该结构域的四个已知等位基因，E-TSR（PNG-MAD 20型）、E-KNG（乌干达-PA型）、Q-KNG（Wellcome型）和Q-TSR（Indo型）。使用单或双交叉重组事件，我们预测了该抗原其他等位基因的存在。预测的等位基因的存在下，从肯尼亚西部的寄生虫分离株，进行了横截面和纵向研究。在10个预测的等位基因中，我们发现了3个新的等位基因：E-KSG-L（Kenya-1型）、E-KSR-L（Kenya-2型）和E-KNG-F（Kenya-3型）。这项研究的结果表明，有可能预测自然寄生虫种群遗传组成的复杂性。(C)1998 Elsevier Science B. V.保留所有权利。
The 19-kDa antigenic domain of Plasmodium falciparum merozoite surface protein (MSP)-1 is a potential malaria vaccine candidate. Based on the amino acid substitution, four known alleles, E-TSR (PNG-MAD20 type), E-KNG (Uganda-PA type), Q-KNG (Wellcome type), and Q-TSR (Indo type) of this domain have been identified. Using single or double crossover recombinational events, we predicted the existence of additional alleles of this antigen. The presence of the predicted alleles was determined in parasite isolates from western Kenya, by undertaking a cross-sectional and a longitudinal study. Of the ten predicted alleles, we have revealed the presence of three new alleles: E-KSG-L (Kenya-1 type); E-KSR-L (Kenya-2 type); and E-KNG-F (Kenya-3 type). The results of this study suggest that it may be possible to predict the complexity of the genetic makeup of natural parasite populations. (C) 1998 Elsevier Science B.V. All rights reserved.