The BarA-UvrY two-component system regulates virulence in avian pathogenic Escherichia coli O78:K80:H9
The BarA-UvrY two-component system regulates virulence in avian pathogenic Escherichia coli O78:K80:H9
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DOI：
10.1128/iai.00412-06
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发表时间：
2006-08-01
影响因子：
3.1
通讯作者：
Mukhopadhyay, Suman
中科院分区：
文献类型：
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作者：
Herren, Christopher D.;Mitra, Arindam;Mukhopadhyay, Suman
已知大肠杆菌中的BarA-UvrY双组分系统（TCS）调节许多表型性状。体外和体内试验，包括鸡胚致死试验，表明该TCS调节禽致病性E.大肠杆菌（APEC）血清型O 78：K 80：H9。一些毒力决定因素，如粘附，侵入，在组织内持续存在，在巨噬细胞内存活，并抵抗血清补体的杀菌作用的能力，在缺乏barA或uvrY基因的突变体中受到损害。降低的毒力是由于下调I型和巴氏菌毛，减少胞外多糖的生产，并增加对氧化应激的敏感性。我们的研究结果表明，BarA-UvrY调节APEC的毒力特性，鸡胚致死率测定可用作替代模型，以确定APEC菌株的毒力决定因素及其调节。
The BarA-UvrY two-component system (TCS) in Escherichia coli is known to regulate a number of phenotypic traits. Both in vitro and in vivo assays, including the chicken embryo lethality assay, showed that this TCS regulates virulence in avian pathogenic E. coli (APEC) serotype O78:K80:H9. A number of virulence determinants, such as the abilities to adhere, invade, persist within tissues, survive within macrophages, and resist bactericidal effects of serum complement, were compromised in mutants lacking either the barA or uvrY gene. The reduced virulence was attributed to down regulation of type I and Pap fimbriae, reduced exopolysaccharide production, and increased susceptibility to oxidative stress. Our results indicate that BarA-UvrY regulates virulence properties in APEC and that the chicken embryo lethality assay can be used as a surrogate model to determine virulence determinants and their regulation in APEC strains.