Biochemical changes between normal and BCC tissue: a FT-Raman study
Biochemical changes between normal and BCC tissue: a FT-Raman study
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正常组织和基底细胞癌组织之间的生化变化：FT-拉曼研究
DOI：
10.1117/12.476884
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
E. Munin
中科院分区：
文献类型：
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作者：
Lilian de Oliveira Nunes;A. Martin;L. Silveira;M. Zampieri;E. Munin
这项工作的主要目的是使用FT-拉曼光谱检测良性和基底细胞癌（BCC）皮肤组织之间的光谱变化。这些光谱变化可以为我们提供有关这两种组织之间生化变化的重要信息。癌症的早期发现仍然是临床肿瘤学的一个巨大挑战。近来，拉曼光谱学已被用于皮肤病变检测。FT-拉曼光谱是一种现代分析工具，其用于癌症诊断将为患者带来几个优点，例如真实的时间和侵入性较小的诊断。我们用傅立叶变换拉曼光谱分析了八组组织病理学诊断为基底细胞癌的样本，并与五组诊断为良性组织的样本进行了比较。我们发现这些样品的光谱差异主要在1220- 1300 cm ~（-1）和1640- 1680 cm ~（-1）的位移区。这些振动带分别对应于酰胺III和酰胺I振动。对所有13个样本应用主成分分析可以以100%的灵敏度和特异性识别组织类型。
The primary objective of this work was to use FT-Raman spectroscopy to detect spectral changes between benign and basal cell carcinoma (BCC) skin tissues. Those spectral changes can give us important information about the biochemical alterations between these two types of tissues. The early cancer detection stills a great challenge in clinical oncology. Recently, Raman spectroscopy has been used for skin lesion detection. FT-Raman spectroscopy is a modern analytical tool and its use for cancer diagnosis will lead to several advantages for the patient as, for example, real time and less invasive diagnosis. We have analyzed by FT-Raman eight sets of samples histopathologically diagnosed as BCC and made a comparison with five sets of samples diagnosed as benign tissue. We have found that the main spectral differences between these samples were in the shift region of 1220-1300cm-1 and 1640-1680cm-1. These vibration bands correspond to the amide III and to the amide I vibrations, respectively. Principal component analysis applied over all 13 samples could identify tissue type with 100% of sensitivity and specificity.