Measuring the Local Velocity along Transition Paths during the Folding of Single Biological Molecules
Measuring the Local Velocity along Transition Paths during the Folding of Single Biological Molecules
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DOI：
10.1103/physrevlett.121.018102
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发表时间：
2018-07-06
影响因子：
8.6
通讯作者：
Woodside, M. T.
中科院分区：
文献类型：
--
作者：
Neupane, Krishna;Hoffer, Noel Q.;Woodside, M. T.
过渡路径是折叠反应中最有趣的部分，但研究很少。利用光镊测量了DNA发夹折叠过程中沿着跃迁路径的局部速度。速度分布符合扩散理论，产生扩散系数。我们使用平均速度来计算过渡态理论（TST）中的传输因子，发现观察到的速率比TST预测的慢10（5）倍。这项工作量化的重要性，障碍recrossing事件，并突出了折叠的扩散模型的有效性。
Transition paths are the most interesting part of folding reactions but remain little studied. We measured the local velocity along transition paths in DNA hairpin folding using optical tweezers. The velocity distribution agreed well with diffusive theories, yielding the diffusion coefficient. We used the average velocity to calculate the transmission factor in transition-state theory (TST), finding observed rates that were similar to 10(5)-fold slower than predicted by TST. This work quantifies the importance of barrier recrossing events and highlights the effectiveness of the diffusive model of folding.