Long-Term Evaluation of Maxillary Reconstruction by Iliac Bone Graft
Long-Term Evaluation of Maxillary Reconstruction by Iliac Bone Graft
复制标题
髂骨移植重建上颌骨的长期评估
DOI：
10.1097/scs.0b013e31822e5cbd
复制
发表时间：
2011
影响因子：
0.9
通讯作者：
E. Gherlone
中科院分区：
文献类型：
--
作者：
S. Tetè;R. Vinci;S. Zara;V. Zizzari;A. De Carlo;G. Falco;D. Tripodi;A. Cataldi;C. Mortellaro;E. Gherlone
本研究的目的是分析在移植后4个月（T1）和10年（T2），与自体髂嵴骨（T0）相比，髂嵴骨移植重建的上颌骨部位发生的组织学和免疫组化变化。通过光学显微镜，T1样本显示组织良好的成骨细胞和细胞外基质（ECM），以及结缔组织周围区域，其中可以区分离散数量的血管。在长时间随访（T2）后从重建区域获得的样品的特征在于均匀矿化的ECM，具有含有骨细胞的空腔，而T0样品公开了矿化基质和骨髓。免疫组化法检测基质金属蛋白酶2（MMP-2）、血管内皮生长因子（VEGF）、诱导型一氧化氮合酶（iNOS）和骨唾液蛋白（BSP）的表达。MMP-2和VEGF在T1中的表达均显著增加，而在T2中的表达降低。另外，BSP的表达，这是在原生髂嵴骨（T0），在T1和T2样本中大大降低。此外，iNOS表达和凋亡细胞核百分比（TUNEL分析）的变化似乎有类似的趋势，T1增加，T2降低。4个月后，髂嵴骨移植重建部位显示矿化核不强烈，如BSP低表达所示，而新血管增殖和主动重塑现象正在发展。移植后较长时间（T2），BSP表达沿着降低，MMP-2、VEGF和iNOS也降低，表明移植物仅存在弱排列现象。
The aim of this study was to analyze histologic and immunohistochemical modifications taking place in maxillary sites reconstructed by iliac crest bone grafts, 4 months (T1) and 10 years (T2) after grafting, in comparison to native iliac crest bone (T0). By light microscopy, T1 samples showed well-organized osteoblasts and extracellular matrix (ECM), and surrounding areas of connective tissue where a discrete number of blood vessels can be distinguished. Samples obtained from reconstructed areas after a long period of follow-up (T2) were characterized by uniform mineralized ECM with cavities containing osteocytes, whereas T0 samples disclosed both mineralized matrix and bone marrow. Matrix metalloproteinase 2 (MMP-2), vascular endothelial growth factor (VEGF), inducible nitric oxide synthase (iNOS), and bone sialoprotein (BSP) expressions were investigated by immunohistochemical analysis. Both MMP-2 and VEGF showed significantly increased expression in T1, with respect to T0, and lowered in T2. Otherwise, BSP expression, which was elevated in native iliac crest bone (T0), was deeply decreased in T1 and T2 samples. Moreover, changes in iNOS expression and in apoptotic cell nuclei percentages (TUNEL analysis) seemed to have a similar trend, increasing in T1 and lowering in T2. After a period of 4 months, iliac crest bone graft-reconstructed sites show mineralizing nuclei not strongly represented, as suggested by BSP lower expression, whereas new blood vessel proliferation and active remodeling phenomena are developing. After a long period from the grafting (T2), BSP expression decreases along with MMP-2, VEGF, and iNOS, suggesting the presence of only weak arrangement phenomena of the graft.
影响因子：
2.7
作者：
Darland, DC;Massingham, LJ;D'Amore, PA
通讯作者：
D'Amore, PA
DOI：
10.1152/ajpheart.00133.2005
发表时间：
2006-02-01
影响因子：
4.8
作者：
Kamba, T;Tam, BYY;McDonald, DM
通讯作者：
McDonald, DM