Ultrastructure, chemistry and mineralogy of the growing shell of the European abalone Haliotis tuberculata
Ultrastructure, chemistry and mineralogy of the growing shell of the European abalone Haliotis tuberculata
复制标题
DOI：
10.1016/j.jsb.2010.05.012
复制
发表时间：
2010-09-01
影响因子：
3
通讯作者：
Huchette, Sylvain
中科院分区：
文献类型：
--
作者：
Auzoux-Bordenave, Stephanie;Badou, Aicha;Huchette, Sylvain
开始了一项涵盖海洋腹足类Haliotis tuberculata整个生命周期的贝壳形成综合研究。利用偏光显微镜、扫描电镜、能谱仪和红外光谱仪对贝壳的微观结构、化学成分和矿物学特征进行了研究。担轮幼虫和面盘幼虫的SEM图像显示了从最初的壳场到后期钙化的原壳的不同阶段的壳生长。横截面揭示了微结构的生物矿物的安排，显示渐进矿化的有机原海螺前变态。为了获得更多的矿物组成信息，沿着开发阶段进行了EDX分析和红外光谱分析。结果表明，早期原贝壳主要由无定形碳酸钙组成，而面盘阶段则逐渐结晶为文石。后变质壳包含两个不同的部分，原来的原海螺支持新的少年壳的特点是一个显着的雕塑图案。仔、稚鲍的贝壳基本上由文石构成。(C)2010年爱思唯尔公司All rights reserved.
An integrated study of shell formation was initiated covering the entire life cycle of the marine gastropod Haliotis tuberculata. Shell microstructure, chemistry and mineralogy were investigated by polarized microscopy, scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDX) and infra-red (IR) spectroscopy. SEM images of trochophore and veliger larvae showed the different stages of shell growth from the initial shell field to the late calcified protoconch. Cross-sections revealed the microstructural arrangement of biominerals, showing the progressive mineralization of the organic protoconch prior to metamorphosis. To gain more information on mineralogical composition, EDX analyses and IR spectroscopy were performed along the development stages. The results demonstrated that early protoconch was mostly composed of amorphous calcium carbonate, while veliger stages showed a gradually crystallization under the form of aragonite. Post-metamorphic shell contained two distinct parts, the original protoconch supporting the new juvenile shell characterized by a marked sculptural pattern. The shells from post-larval and juvenile abalones were essentially made of aragonite. (C) 2010 Elsevier Inc. All rights reserved.