Molecular diagnosis of group B coltiviruses infections
Molecular diagnosis of group B coltiviruses infections
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DOI：
10.1016/s0166-0934(99)00055-5
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发表时间：
1999-08-01
影响因子：
3.1
通讯作者：
de Lamballerie, X
中科院分区：
文献类型：
--
作者：
Billoir, F;Attoui, H;de Lamballerie, X
Coltivirus属的B组包括从印度尼西亚和中国收集的人、蜱或蚊子的分离物。B1亚群包括毒株JKT/dsR-7075和B2亚群毒株JKTdsR-6423、JKT/dsR-6969、JKT/dsR-7043和班纳病毒。数据描述为基于PCR的诊断感染的B组大肠杆菌病毒。根据第7、9和12病毒片段的序列设计引物组，并开发RT-PCR检测方法。共有引物允许检测亚组1或2的所有已知分离株。病毒株可使用B2 a或B2b型特异性引物或基于扩增产物的长度进一步表征。当使用来自环状病毒或A组结肠炎病毒的RNA时，所有引物均给出阴性结果。这些引物允许在疾病的急性阶段检测受感染的小鼠血液中的B组Coltiviruses-RNA。因此，它们可用于人类感染的诊断。(C)1999 Elsevier Science B. V.保留所有权利。
The group-B of genus Coltivirus encompasses isolates from humans, ticks or mosquitoes collected in Indonesia and China. Subgroup-B1 includes the strain JKT/dsR-7075 and subgroup-B2 strains JKTdsR-6423, JKT/dsR-6969, JKT/dsR-7043 and the Banna virus. Data are described for the PCR-based diagnosis of infection by group B coltiviruses. Sets of primers were designed from the sequences of the 7th, 9th and 12th viral segments and RT-PCR assays were developed Consensus primers permitted the detection of all known isolates of subgroup 1 or 2. Viral strains could be characterised further using primers specific for type B2a or B2b, or based on the length of the amplification products. All primers gave negative results when using RNAs from Orbiviruses or Group-A coltiviruses. These primers permitted the detection of Group-B coltiviruses-RNA in infected mouse blood at the acute stage of the disease. Accordingly, they could be used for the diagnosis of infection in humans. (C) 1999 Elsevier Science B.V. All rights reserved.