Tyrosinase inhibitory effect and inhibitory mechanism of tiliroside from raspberry
Tyrosinase inhibitory effect and inhibitory mechanism of tiliroside from raspberry
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DOI：
10.1080/14756360802694252
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发表时间：
2009-10-01
影响因子：
5.6
通讯作者：
Tao, Xin-yi
中科院分区：
文献类型：
--
作者：
Lu, Yan-hua;Chen, Juan;Tao, Xin-yi
铁洛苷对蘑菇酪氨酸酶的单酚酶和二酚酶活性均有抑制作用。蘑菇酪氨酸酶催化酪氨酸氧化的滞后时间明显延长；0.337 mM的铁洛苷使滞后时间由46.7s延长至431.5 s。动力学分析表明，在K值分别为0.052 mM和0.26 mM时，铁力苷是单酚醇酶和二酚酶的竞争性抑制剂。此外，在0.168 mM时，替里罗苷对B16小鼠黑色素瘤细胞胞内酪氨酸酶活性的抑制作用为34.5% (p < 0.05)，对黑色素生成的抑制作用为54.1% (p < 0.05)，而相同浓度下熊果苷对细胞酪氨酸酶活性的抑制作用为9.1%，对黑色素生成的抑制作用为29.5%。结果表明，铁力罗苷是一种有效的酪氨酸酶抑制剂，可作为皮肤增白剂和色素沉着剂。
Tiliroside was found to inhibit both monophenolase and diphenolase activity of mushroom tyrosinase. The lag time of tyrosine oxidation catalyzed by mushroom tyrosinase was obviously lengthened; 0.337 mM of tiliroside resulted in the lag time extension from 46.7s to 435.1s. A kinetic analysis shown that tiliroside was a competitive inhibitor for monophenolase and diphenolase with K, values of 0.052 mM and 0.26 mM, respectively. Furthermore, tiliroside showed 34.5% (p < 0.05) inhibition of intracellular tyrosinase activity and 54.1% (p < 0.05) inhibition of melanin production with low cytotoxicity on B16 mouse melanoma cells at 0.168 mM. In contrast, arbutin displayed 9.1% inhibition of cellular tyrosinase activity and 29.5% inhibition of melanin production at the same concentration. These results suggested that tiliroside was a potent tyrosinase inhibitor and might be used as a skin-whitening agent and pigmentation medicine.