THE LOSS OF PHENOTYPIC TRAITS BY DIFFERENTIATED CELLS IN VITRO .1. DEDIFFERENTIATION OF CARTILAGE CELLS
THE LOSS OF PHENOTYPIC TRAITS BY DIFFERENTIATED CELLS IN VITRO .1. DEDIFFERENTIATION OF CARTILAGE CELLS
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DOI：
10.1073/pnas.46.12.1533
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发表时间：
1960-01-01
影响因子：
11.1
通讯作者：
HOLTZER, S
中科院分区：
文献类型：
--
作者：
HOLTZER, H;ABBOTT, J;HOLTZER, S
用胰酶从胚胎软骨中解放出来的软骨细胞立即进行器官培养，继续合成硫酸软骨素，并将无机S35掺入到酯结合的硫酸盐中。在抑制分化但促进细胞增殖的条件下培养后，这些细胞在器官培养时，(1)不能分化为可识别的软骨细胞，(2)不能合成硫酸软骨素，(3)不能将S35结合到酯结合的硫酸盐中。去分化的软骨细胞可能分化为脂肪细胞。
Chondrocytes liberated from embryonic cartilage by means of trypsin and immediately organ-cultured continue to synthesize chondrotin sulfate and incorporate inorganic S35 into ester-bound sulfate. After being cultured under conditions that suppress differentiation but encourage cell multiplication, these cells, when organ-cultured, (1) fail to differentiate into recognizable chondrocytes, (2) fail to synthesize chondroitin sulfate, and (3) fail to incorporate S35 into ester-bound sulfate. Dedifferentiated cartilage cells may differentiate into fat cells.