YB-1 relocates to the nucleus in adenovirus-infected cells and facilitates viral replication by inducing E2 gene expression through the E2 late promoter
YB-1 relocates to the nucleus in adenovirus-infected cells and facilitates viral replication by inducing E2 gene expression through the E2 late promoter
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DOI：
10.1074/jbc.m106955200
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发表时间：
2002-03-22
影响因子：
4.8
通讯作者：
Royer, HD
中科院分区：
文献类型：
--
作者：
Holm, PS;Bergmann, S;Royer, HD
腺病毒早期蛋白E1 A和E1 B-55 kDa是病毒DNA复制的关键调节因子，并且认为E1 B-55 kDa靶向p53对于该过程是必需的。在这里，我们已经确定了一个以前未被认识到的功能E1 B腺病毒复制。我们发现E1 B-55 kDa参与将转录因子YB-1靶向至腺病毒5型感染的细胞的细胞核，在那里它与被认为是病毒转录和复制位点的病毒包涵体相关。我们发现，YB-1通过E2晚期启动子促进E2基因表达，从而在感染后期控制E2基因活性。用含有YB-1转基因的El-负腺病毒载体证明了YB-1对腺病毒复制的作用。在感染的细胞中，AdYB-1有效地复制并产生感染性子代颗粒。因此，腺病毒E1 B-55 kDa蛋白和宿主细胞因子YB-1共同作用，以促进腺病毒在感染后期的复制。
The adenovirus early proteins E1A and E1B-55kDa are key regulators of viral DNA replication, and it was thought that targeting of p53 by E1B-55kDa is essential for this process. Here we have identified a previously unrecognized function of E1B for adenovirus replication. We found that E1B-55kDa is involved in targeting the transcription factor YB-1 to the nuclei of adenovirus type 5-infected cells where it is associated with viral inclusion bodies believed to be sites of viral transcription and replication. We show that YB-1 facilitates E2 gene expression through the E2 late promoter thus controlling E2 gene activity at later stages of infection. The role of YB-1 for adenovirus replication was demonstrated with an El-minus adenovirus vector containing a YB-1 transgene. In infected cells, AdYB-1 efficiently replicated and produced infectious progeny particles. Thus, adenovirus E1B-55kDa protein and the host cell factor YB-1 act jointly to facilitate adenovirus replication in the late phase of infection.