RAPID ESTIMATION OF THE AMYLOSE AMYLOPECTIN RATIO IN SMALL AMOUNTS OF TUBER AND LEAF TISSUE OF THE POTATO
RAPID ESTIMATION OF THE AMYLOSE AMYLOPECTIN RATIO IN SMALL AMOUNTS OF TUBER AND LEAF TISSUE OF THE POTATO
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DOI：
10.1007/bf02365532
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发表时间：
1988-06-01
期刊：
影响因子：
2.9
通讯作者：
FEENSTRA, WJ
中科院分区：
文献类型：
--
作者：
HOVENKAMPHERMELINK, JHM;DEVRIES, JN;FEENSTRA, WJ
少量的马铃薯块茎和叶组织在高氯酸中提取。用I2-KI溶液染色后，测量618和550 nm处的吸光度。直链淀粉/支链淀粉的比率可以通过使用公式或图表从吸光度的比率中估计出来，在公式或图表中引入了这两种化合物的特定吸收。不需要称重样品。该方法可用于微小块茎淀粉组成的测定，尤其适用于淀粉组成发生变化的突变株的检测。
Small amounts of potato tuber and leaf tissues are extracted in perchloric acid. After staining with I2-KI solution absorbancies at 618 and 550 nm are measured. The amylose/amylopectin ratio can be estimated from the ratio of the absorbancies by using a formula or a graph in which the specific absorptions of the two compounds are introduced. Weighing of samples is not required. The method can be used for the estimation of the starch composition of minitubers and is especially suitable for the detection of mutants with an altered starch composition.