Differences in transport of fatty acids and expression of fatty acid transporting proteins in adipose tissue of obese black and white women
Differences in transport of fatty acids and expression of fatty acid transporting proteins in adipose tissue of obese black and white women
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DOI：
10.1152/ajpendo.00194.2005
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发表时间：
2006-01-01
影响因子：
5.1
通讯作者：
Barakat, HA
中科院分区：
文献类型：
--
作者：
Bower, JF;Davis, JM;Barakat, HA
肥胖黑人和白人女性脂肪组织中脂肪酸转运和脂肪酸转运蛋白表达的差异。Am J Physiol Endocrinol Metab290：E87-E91,2006年；DOI：10.1152/ajendo。00194.2005。-我们已经报道，非裔美国女性(AAW)大网膜脂肪组织(而非皮下脂肪组织)合成甘油三酯(TG)的比率高于高加索女性(CAW)。这项研究的目的是探索这种增长背后的潜在机制。为此，我们测定了AAW和CAW大网膜脂肪中TG合成途径中关键酶的活性、脂肪细胞对脂肪酸的摄取速率、脂肪酸转运蛋白FAT/CD36和FATPmRNA和蛋白质水平以及PPAR-γ的mRNA和蛋白质水平。结果表明：1)磷酸果糖激酶、3-磷酸甘油脱氢酶、甘油-3-磷酸脱氢酶或二酰甘油酰基转移酶的活性没有差异；2)AAW的脂肪细胞对脂肪酸的摄取率较高；3)AAW的脂肪中CD36和FATP4的mRNA和蛋白水平增加；4)PPAR-γ的mRNA和蛋白水平增加，从而刺激CD36和FATP4的表达。这些结果提示，由大网膜脂肪组织中运输蛋白的过度表达所介导的脂肪酸转运增加，可能是导致小儿麻痹症肥胖发生率较高的原因之一。
Differences in transport of fatty acids and expression of fatty acid transporting proteins in adipose tissue of obese black and white women. Am J Physiol Endocrinol Metab 290: E87 - E91, 2006; doi: 10.1152/ajpendo. 00194.2005. - We have reported that the rate of de novo triglyceride (TG) synthesis by omental, but not subcutaneous, adipose tissue was higher in African-American women (AAW) than in Caucasian women (CAW). The purpose of this study was to explore the potential mechanisms underlying this increase. Toward that end, we determined the activities of key enzymes in the pathway of TG synthesis, the rates of uptake of fatty acids by adipocytes, mRNA and protein levels of the fatty acid-transporting proteins FAT/CD36 and FATP, and mRNA and protein levels of PPAR gamma in omental fat of AAW and CAW. The results showed 1) no difference in the activity of phosphofructokinase, glycerol-3-phosphate dehydrogenase, or diacylglycerol acyltransferase; 2) a higher rate of fatty acid uptake by adipocytes of the AAW; 3) an increase in the mRNA and protein levels of CD36 and FATP4 in the fat of the AAW; and 4) an increase in the mRNA and protein levels of PPAR gamma, which can stimulate the expression of CD36 and FATP. These results suggest that the increase in the transport of fatty acid, which is mediated by the overexpression of the transport proteins in the omental adipose tissue of the AAW, might contribute to the higher prevalence of obesity in AAW.