Atomic force microscopic measurement of the mechanical properties of intact endothelial cells in fresh arteries
Atomic force microscopic measurement of the mechanical properties of intact endothelial cells in fresh arteries
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DOI：
10.1007/bf02513342
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发表时间：
1999-07-01
影响因子：
3.2
通讯作者：
Hayashi, K
中科院分区：
文献类型：
--
作者：
Miyazaki, H;Hayashi, K
通过原子力显微镜（AFM）测定了兔腹主动脉和主动脉分叉内侧壁和外侧壁活内皮细胞的力学特性，重点是位置差异。用指数函数F = a（exp（B delta）-1）表示细胞的力（F）-压痕（delta）曲线，其中a和B是常数。参数B和c（= ab）分别表示模量变化率和初始模量。F-S曲线a的斜率和参数c在内侧壁高于其他部位，这是由于内侧壁内皮细胞中有丰富的应力纤维。三个位置之间的参数B无差异。这些结果表明，内皮细胞在主动脉分叉的内侧壁比在其他区域更坚硬。
Mechanical properties of living endothelial cells in the abdominal aortas and in the medial and lateral wall of aortic bifurcations obtained from rabbits were determined by means of an atomic force microscope (AFM), focusing on the locational differences. Force (F)-indentation (delta) curves of the cells were expressed by an exponential function: F = a(exp(b delta)-1), where a and b are constants. The parameters b and c(= ab) represent the rate of modulus change and initial modulus, respectively. The slope of F-S curves a and the parameter c were higher in the medial wall than in the other sites, which is attributable to abundant stress fibres in endothelial cells in the medial wall. There were no differences in the parameter b among the three locations. These results indicate that endothelial cells are stiffer in the medial wall of aortic bifurcation than in the other regions.