Relationship between meat structure, water mobility, and distribution: A low-field nuclear magnetic resonance study
Relationship between meat structure, water mobility, and distribution: A low-field nuclear magnetic resonance study
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DOI：
10.1021/jf010738f
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发表时间：
2002-02-13
影响因子：
6.1
通讯作者：
Andersen, HJ
中科院分区：
文献类型：
--
作者：
Bertram, HC;Purslow, PP;Andersen, HJ
采用核磁共振（NMR）测量方法对猪肉最长肌进行了预处理，研究了肉的微观结构与核磁共振T-2弛豫之间的关系。分布指数分析的核磁共振T-2弛豫数据显示存在三个不同的水种群：T-2b， T-21和T-22。考虑到s型关系，T-21时间常数与肌节长度（r = 0.84）和i带和A带区域肌丝点阵体积计算比（r = 0.84）之间存在高度显著的相关性。结果表明，T-21时间常数主要由肌丝晶格结构决定，因此有力地支持了先前提出的理论，即T-21种群对应于位于高度组织的肌纤维蛋白基质中的水，包括肌动蛋白和肌球蛋白丝结构。T-22种群与持水能力（WHC）之间也存在高度相关性（r = 0.76）。这表明WHC主要是由松散结合的肌纤维外水的数量决定的。然而，在相关分析中加入T-21时间常数后，NMR T-2参数与WHC的相关性进一步增强（r = 0.84）。这意味着滴水损失的形成是一个持续的过程，涉及水从肌原纤维转移到细胞外空间，并受到肌肉组织内几个组织水平的结构特征的影响。这项研究证明了核磁共振T-2弛豫作为一种有效的技术的优势，可以进一步了解肉的微观结构、其WHC、水迁移率和水分布之间的关系。
Nuclear magnetic resonance (NMR) measurements were carried out on pork longissimus muscle, which pre rigor had been manipulated to various muscle lengths, to investigate the relationship between the microstructure of meat and the NMR T-2 relaxation. Distributed exponential analysis of the NMR T-2 relaxation data revealed the existence of three distinct water populations: T-2b, T-21, and T-22. A high, significant correlation was found between the T-21 time constant and the sarcomere length (r = 0.84) and calculated ration of myofilament lattice volume in the I-band and A-band regions, respectively (r = 0.84), considering sigmoid relationships. The result implies that the T-21 time constant mainly is determined by the structure of the myofilament lattice and so strongly supports a previously proposed theory that the T-21 population corresponds to water located within a highly organized myofibrillar protein matrix including actin and myosin filament structures, A high correlation was also found between the T-22 population and the water-holding capacity (WHC) (r = 0.76), which suggests that the WHC is mainly determined by the amount of loosely bound extramyofibrillar water. However, the correlation between NMR T-2 parameters and WHC was further increased (r = 0.84) by including the T-21 time constant in the correlation analysis. This implies that the formation of drip loss is an ongoing process involving the transfer of water from myofibrils to the extracellular space and is affected by structural features at several levels of organization within the muscle tissue. This study demonstrates the advantages of NMR T-2 relaxation as an effective technique for obtaining further understanding of the relationship between the microstructure of meat, its WHC, water mobility, and water distribution.