Evidence for a direct effect of LRF and TRF on single unit activity in the rostral hypothalamus
Evidence for a direct effect of LRF and TRF on single unit activity in the rostral hypothalamus
复制标题
LRF 和 TRF 对下丘脑头端单个单位活动有直接影响的证据
作者：
R. Dyer;R. Dyball
脑垂体和其他内分泌腺产生的许多激素影响大脑的电活动。现在越来越多的人意识到，调节垂体前叶激素分泌的一些下丘脑释放因子本身可能对中枢神经系统有类似的影响2 -4。促甲状腺激素释放因子[TRF;（pyro）Glu-His-Pro（NH 2）] 5，6和促黄体生成激素释放因子[LRF;（pyro）Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly（NH 2）]7的氨基酸序列已经完全确定，并且这些合成产生的具有高比活性的多肽容易获得。进行将要描述的实验以确定LRF和/或TRF是否对前脑中神经元的电活动具有直接影响。从下丘脑和大脑皮层的单个细胞记录动作电位，并通过微离子电渗法将LRF和TRF施加于它们。还测试了一些神经元对催产素（一种类似大小的生物活性肽）的敏感性。
MANY of the hormones produced by the pituitary and other endocrine glands influence the electrical activity of the brain (for recent references see Dyer1). There is now a growing awareness that some of the hypothalamic releasing factors, which regulate the secretion of hormones from the anterior pituitary gland, may themselves have a similar influence upon the central nervous system2–4. The amino acid sequences for both thyrotrophin releasing factor [TRF; (pyro) Glu-His-Pro (NH2)]5,6 and luteinising hormone releasing factor [LRF; (pyro) Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly (NH2)]7 have been fully established and these polypeptides, produced synthetically and of high specific activity, are readily available. The experiments to be described were undertaken to establish whether LRF and/or TRF have a direct effect upon the electrical activity of neurones in the forebrain. Action potentials were recorded from single cells in the hypothalamus and cerebral cortex and LRF and TRF applied to them by microiontophoresis. The sensitivity of some of the neurones to oxytocin (a biologically active peptide of similar size) was also tested.