Molecular cloning of the Xenopus c-met/hepatocyte growth factor receptor and its regional expression during early development.
Molecular cloning of the Xenopus c-met/hepatocyte growth factor receptor and its regional expression during early development.
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非洲爪蟾 c-met/肝细胞生长因子受体的分子克隆及其早期发育过程中的区域表达。
DOI：
10.1093/oxfordjournals.jbchem.a021513
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发表时间：
1996
影响因子：
2.7
通讯作者：
T. Nakamura
中科院分区：
文献类型：
--
作者：
S. Aoki;K. Takahashi;K. Matsumoto;T. Nakamura
肝细胞生长因子（HGF）是c-Met受体酪氨酸激酶的配体，调控多种细胞的生长、迁移和形态发生，在胚胎器官发生和器官再生中起重要作用。HGF在非洲爪蟾中结构保守，在爪蟾神经胚和尾胚中均有表达。本研究克隆了爪蟾Met/ HGF受体的互补DNA，并分析了其在胚胎发生过程中的表达。Xenopus c-met的开放阅读框长4125个碱基对，编码一种含有1375个氨基酸的多肽。推导出的爪蟾Met蛋白氨基酸序列与人相同度为63%，胞内区域酪氨酸激酶结构域具有特殊的同源性，与人同源性超过90%。在爪蟾胚胎发生过程中，c-met mRNA从原胚期到神经胚期表达水平较高，而HGF mRNA在神经胚期早期表达水平较高。全株原位杂交表明，c-met mRNA特异性定位于前肠区、尾芽间充质组织和神经胚的神经组织。因此，c-Met在许多物种中都是高度保守的分子，它的配体HGF也是如此。HGF-Met系统可能参与爪蟾胚胎早期多器官发生。
Hepatocyte growth factor (HGF), a ligand for c-Met receptor tyrosine kinase, regulates the cell growth, migration and morphogenesis of a wide variety of cells, and plays important roles in organogenesis in embryos as well as regeneration of organs. HGF is structurally conserved in Xenopus laevis, and the Xenopus HGF gene is expressed in neurula and tailbud embryos. In the present work, we cloned complementary DNA for the Xenopus Met/ HGF receptor and analyzed its expression during embryogenesis. The open reading frame of Xenopus c-met is 4,125 base pairs long and encodes a putative polypeptide of 1,375 amino acids. The deduced amino acid sequences of the Xenopus and human Met proteins are 63% identical, the tyrosine kinase domains in the intracellular regions showing particular homology, over 90% identity in the amino acid sequences between Xenopus and human. During Xenopus embryogenesis, c-met mRNA was expressed at a high level from the gastrula to the neurula stage, while HGF mRNA expression was seen from the early neurula stage. Whole mount in situ hybridization showed that c-met mRNA was specifically localized in the foregut region, mesenchymal tissue of the tailbud, and neural tissues in neurula embryos. Thus, c-Met is a highly conserved molecule in a wide range of species, as is its ligand, HGF. The HGF-Met system may be involved in early multiple organogenesis in Xenopus embryos.