Ribonuclease activity associated with the 60S ribosome-inactivating proteins ricin A, phytolaccin and Shiga toxin.
Ribonuclease activity associated with the 60S ribosome-inactivating proteins ricin A, phytolaccin and Shiga toxin.
复制标题
核糖核酸酶活性与 60S 核糖体失活蛋白蓖麻毒素 A、植胶素和志贺毒素相关。
DOI：
10.1016/0006-291x(85)90498-x
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发表时间：
1985
影响因子：
3.1
通讯作者：
Colinas,RJ
中科院分区：
文献类型：
--
作者：
Obrig,TG;Moran,TP;Colinas,RJ
所有纯化的核糖体失活蛋白蓖麻蛋白A、植痘蛋白和志贺毒素在5S或5.8S rRNA底物上均表现出核糖核酸酶活性。这些毒素产生可复制的RNA片段模式，每种毒素都不同，而单一毒素的多种制剂产生类似的RNA片段模式。志贺毒素的热失活特征与RNase和蛋白质合成抑制活性相同。这些数据是第一个表明核糖体失活催化毒素，除了α -sarcin，表现出rna酶活性。这些结果提示RNase活性可能是蓖麻毒素、植痘蛋白和志贺毒素蛋白催化核糖体失活的原因。
All purified preparations of the ribosome-inactivating proteins ricin A, phytolaccin and Shiga toxin were shown to exhibit ribonuclease activity with 5S or 5.8S rRNA substrates. These toxin species generated reproducible patterns of RNA fragments distinct for each toxin species while multiple preparations of a single toxin species yielded similar RNA fragment patterns. The heat inactivation profile of Shiga toxin was identical for its RNase and protein synthesis inhibitory activities. These data are the first to indicate that the ribosome-inactivating catalytic toxins, in addition to alpha-sarcin, exhibit RNase activity. These results suggest RNase activity may be responsible for ribosome-inactivation catalyzed by ricin, phytolaccin and Shiga toxin proteins.