Expression of membrane and nuclear melatonin receptors in mouse peripheral organs
Expression of membrane and nuclear melatonin receptors in mouse peripheral organs
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DOI：
10.1016/j.lfs.2003.08.046
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发表时间：
2004-03-19
期刊：
影响因子：
6.1
通讯作者：
Calvo, JR
中科院分区：
文献类型：
--
作者：
Naji, L;Canillo-Vico, A;Calvo, JR
以前的研究表明，褪黑激素通过特异性受体起作用，包括MT 1和MT 2膜受体以及属于孤儿核受体家族的核受体。因此，本研究的目的是确定褪黑激素受体mRNA是否在小鼠外周组织中表达。为了研究不同受体亚型的表达，我们使用了逆转录聚合酶链反应（RT-PCR）的程序，然后用特异性地高辛标记的探针进行Southern杂交。RT-PCR研究揭示了MT 1膜受体和ROR（α）1核受体在所有检查的外周组织（脑、心、肺、肝和肾）中的存在。Western-blot检测ROR（alpha）1核受体的表达。相反，MT 2膜受体的表达仅在脑和肺中观察到。这些结果表明，褪黑激素，通过其不同亚型的受体，在外周组织功能的神经内分泌调节中发挥作用。(C)2004爱思唯尔公司All rights reserved.
Previous studies have shown that melatonin acts through specific receptors, including MT1 and MT2 membrane receptors as well as a nuclear receptor belonging to the orphan nuclear receptor family. Therefore, the goal of this study was to determine whether melatonin receptors mRNA is expressed in mouse peripheral tissues. To study the different receptors subtype expression, we have used a reverse-transcription polymerase chain reaction (RT-PCR) procedure followed by Southern hybridization with specific digoxigenin-labeled probes. RT-PCR studies revealed the presence of both MT1 membrane receptors and ROR(alpha)1 nuclear receptors in all the peripheral tissues examined (brain, heart, lung, liver and kidney). Moreover, the expression of ROR(alpha)1 nuclear receptors was also demonstrated by Western-blot. In contrast, expression of MT2 membrane receptors was only observed in brain and lung. These results suggest that melatonin, acting through its different subtypes receptors, plays a role in the neuroendocrine regulation of peripheral tissues function. (C) 2004 Elsevier Inc. All rights reserved.