Engrailed and polyhomeotic maintain posterior cell identity through cubitus-interruptus regulation
Engrailed and polyhomeotic maintain posterior cell identity through cubitus-interruptus regulation
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DOI：
10.1016/j.ydbio.2004.05.020
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发表时间：
2004-08-15
影响因子：
2.7
通讯作者：
Maschat, F
中科院分区：
文献类型：
--
作者：
Chanas, G;Lavrov, S;Maschat, F
在果蝇中，将细胞细分为隔室需要在后部细胞中表达engrailed（en）和hedgehog（hh），以及在前部细胞中表达cubitus-interruptus（ci）。而后部细胞表达hh，只有前部细胞有能力对hh信号作出反应，因为在这些细胞中存在ci表达。在这里，我们表明，enrailed和polyhomeotic（ph），Polycomb组（PcG）基因的成员，同时采取行动，以保持在发展过程中的后车厢抑制CI。使用染色质免疫沉淀（ChIP），我们确定了一个1 kb的基因组片段位于4 kb的CI编码区，负责调控ei的上游。该基因组片段在体内被Polyhomeotic和Engrailed结合。特别是，我们表明，Engrailed是负责建立CI镇压早期胚胎发育过程中，也需要，沿着与Polyhomeotic，以保持抑制CI整个发展。(C)2004爱思唯尔公司All rights reserved.
In Drosophila, the subdivision into compartments requires the expression of engrailed (en) and hedgehog (hh) in the posterior cells and of cubitus-interruptus (ci) in the anterior cells. Whereas posterior cells express hh, only anterior cells are competent to respond to the hh signal, because of the presence of ci expression in these cells. We show here that engrailed and polyhomeotic (ph), a member of the Polycomb Group (PcG) genes, act concomitantly to maintain the repression of ci in posterior compartments during development. Using chromatin immunoprecipitation (ChIP), we identified a 1 kb genomic fragment located 4 kb upstream of the ci coding region that is responsible for the regulation of ei. This genomic fragment is bound in vivo by both Polyhomeotic and Engrailed. In particular, we show that Engrailed is responsible for the establishment of ci repression early during embryonic development and is also required, along with Polyhomeotic, to maintain the repression of ci throughout development. (C) 2004 Elsevier Inc. All rights reserved.