A mouse model of diet-induced obesity and insulin resistance.
A mouse model of diet-induced obesity and insulin resistance.
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DOI：
10.1007/978-1-61779-430-8_27
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Liao, James K
中科院分区：
文献类型：
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作者：
Wang, Chao-Yung;Liao, James K
肥胖在西方社会已成为流行病。它导致了医疗负担的增加和预期寿命的缩短。肥胖是一种复杂的慢性疾病，涉及数十年的病理生理变化和适应。因此，很难确定人类这一长期过程的确切机制。为了规避这些问题，有几种替代模型可用，包括小鼠基因功能丧失突变、转基因功能获得突变、多基因模型和不同的环境暴露模型。饮食诱导肥胖的小鼠模型已成为了解高脂肪西方饮食与肥胖发展之间相互作用的最重要工具之一。饮食性肥胖模型与近二十年来现代社会中越来越多的高脂肪/高密度食物密切相关，这些食物是导致人类肥胖趋势的主要因素。这个模型已经导致许多肥胖的重要信号的发现，如Akt和mTOR。本章主要介绍了小鼠饮食性肥胖模型的建立方案以及胰岛素抵抗和敏感性的测量方案。
Obesity is reaching pandemic proportions in Western society. It has resulted in increasing health care burden and decreasing life expectancy. Obesity is a complex, chronic disease, involving decades of pathophysiological changes and adaptation. Therefore, it is difficult ascertain the exact mechanisms for this long-term process in humans. To circumvent some of these issues, several surrogate models are available, including murine genetic loss-of-function mutations, transgenic gain-of-function mutations, polygenic models, and different environmental exposure models. The mouse model of diet-induced obesity has become one of the most important tools for understanding the interplay of high-fat Western diets and the development of obesity. The diet-induced obesity model closely mimics the increasingly availability of the high-fat/high-density foods in modern society over the past two decades, which are main contributors to the obesity trend in human. This model has lead to many discoveries of the important signalings in obesity, such as Akt and mTOR. The chapter describes protocols for diet induced-obesity model in mice and protocols for measuring insulin resistance and sensitivity.