Simultaneous determination of nitroimidazoles, benzimidazoles, and chloramphenicol components in bovine milk by ultra-high performance liquid chromatography-tandem mass spectrometry
Simultaneous determination of nitroimidazoles, benzimidazoles, and chloramphenicol components in bovine milk by ultra-high performance liquid chromatography-tandem mass spectrometry
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DOI：
10.1016/j.foodchem.2015.07.033
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发表时间：
2016-02-01
期刊：
影响因子：
8.8
通讯作者：
Xia, Xi
中科院分区：
文献类型：
--
作者：
Wang, Yuanyuan;Li, Xiaowei;Xia, Xi
建立了一种灵敏的、确证性的超高效液相色谱-串联质谱方法，并验证了该方法可用于检测牛乳中23种兽药及其代谢产物（硝基咪唑类、苯并咪唑类和氯霉素类成分）。感兴趣的化合物依次用乙腈和碱化乙腈从牛奶中提取，使用氯化钠诱导液-液分配。提取物在混合模式固相萃取柱上纯化。在电喷雾离子化中使用快速极性切换，单次注射能够在9分钟色谱运行时间内检测带正电荷和带负电荷的分析物。基于基质匹配校准和同位素标记的牛奶内标物的回收率范围为51.7%至101.8%。分析方法的检测限和定量限分别在2-20 ng/kg和5-50 ng/kg范围内。推荐的方法是简单的，具体的，和可靠的常规监测硝基咪唑类，苯并咪唑类和氯霉素成分的牛奶样品。(C)2015爱思唯尔有限公司版权所有。
A sensitive, confirmatory ultra-high performance liquid chromatography-tandem mass spectrometric method was developed and validated to detect 23 veterinary drugs and metabolites (nitroimidazoles, benzimidazoles, and chloramphenicol components) in bovine milk. Compounds of interest were sequentially extracted from milk with acetonitrile and basified acetonitrile using sodium chloride to induce liquid-liquid partition. The extract was purified on a mixed mode solid-phase extraction cartridge. Using rapid polarity switching in electrospray ionization, a single injection was capable of detecting both positively and negatively charged analytes in a 9 min chromatography run time. Recoveries based on matrix-matched calibrations and isotope labeled internal standards for milk ranged from 51.7% to 101.8%. The detection limits and quantitation limits of the analytical method were found to be within the range of 2-20 ng/kg and 5-50 ng/kg, respectively. The recommended method is simple, specific, and reliable for the routine monitoring of nitroimidazoles, benzimidazoles, and chloramphenicol components in bovine milk samples. (C) 2015 Elsevier Ltd. All rights reserved.