BIOGENESIS OF VACCINIA - ISOLATION OF CONDITIONAL LETHAL MUTANTS AND ELECTRON-MICROSCOPIC CHARACTERIZATION OF THEIR PHENOTYPICALLY EXPRESSED DEFECTS
BIOGENESIS OF VACCINIA - ISOLATION OF CONDITIONAL LETHAL MUTANTS AND ELECTRON-MICROSCOPIC CHARACTERIZATION OF THEIR PHENOTYPICALLY EXPRESSED DEFECTS
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DOI：
10.1016/0042-6822(78)90258-1
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发表时间：
1978-01-01
期刊：
影响因子：
3.7
通讯作者：
MCFADDEN, G
中科院分区：
文献类型：
--
作者：
DALES, S;MILOVANOVITCH, V;MCFADDEN, G
描述了用于收集和鉴定 IHD 痘苗合胞体菌株的温度敏感突变体的 4 阶段选择方案。在测试的约 6000 个噬菌斑分离株中，有 90 个被认为在实验上有用，因为它们具有足够的热敏感性和低可逆性。有用的突变体中有七个被证明在 DNA 合成方面存在缺陷。通过电镜检查大多数可接受的突变体，以确定它们在限制温度下复制时表现出的形态发生缺陷。明显的广泛缺陷，即特定突变体的每个特征，根据达到 40 度的发育复杂性，按升序排列为 17 类。 C. 与每个类别相关的缺陷在一系列电子显微照片和随附的描述中进行了举例说明。
A 4-stage selection scheme is described for collecting and identifying temperature-sensitive mutants of a syncytiogenic strain of IHD vaccinia. Among about 6000 plaque isolates tested, 90 were judged to be experimentally useful because of their adequate thermosensitivity and low rate of reversibility. Seven among the useful mutants proved to be defective in DNA synthesis. Most of the acceptable mutants were examined by EM to ascertain the defects in morphogenesis which they manifest when allowed to replicate at the restrictive temperature. The wide spectrum of defects which became evident, each characteristic of a particular mutant, was schematized into an ascending order of 17 categories according to the complexity in development reached at 40.degree. C. The defects associated with each category are exemplified in a series of electron micrographs and accompanying descriptions.