Detection of Interleukin-33 in Serum and Carcinoma Tissue from Patients with Hepatocellular Carcinoma and its Clinical Implications
Detection of Interleukin-33 in Serum and Carcinoma Tissue from Patients with Hepatocellular Carcinoma and its Clinical Implications
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DOI：
10.1177/030006051204000504
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发表时间：
2012-10
影响因子：
1.6
通讯作者：
Ping Zhang;Xingkai Liu;Z. Chu;Ye Jc;Li Kl;Zhuang Wl;Yang Dj;Yuting Jiang
中科院分区：
文献类型：
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作者：
Ping Zhang;Xingkai Liu;Z. Chu;Ye Jc;Li Kl;Zhuang Wl;Yang Dj;Yuting Jiang
目的：探讨细胞间白细胞介素（IL）-33在肝细胞癌（HCC）中的临床意义。研究方法：使用免疫组织化学，这项前瞻性研究比较了60例HCC患者的HCC组织和肿瘤附近正常组织样本中的IL-33蛋白水平，以及6例健康对照的正常肝组织中的IL-33蛋白水平。采用酶联免疫吸附试验（ELISA）检测30例HCC患者和10例健康对照者血清IFN-α、IFN-γ和IL-33水平。比较IL-33免疫组化染色水平与HCC患者淋巴结转移率的关系。结果：IL-33在肝细胞胞浆中呈强阳性表达。HCC中IL-33阳性组织的中位百分比高于正常肝组织样本（邻近肿瘤或来自对照）。肝癌患者术前、术后血清IFN-α、IFN-γ和IL-33水平均高于对照组，有转移者高于无转移者。结论：在HCC患者血清和肝组织中观察到IL-33蛋白水平升高，IL-33可能是监测HCC生长和转移的有用生物学标志物。
Objective: To study the clinical significance of intercellular interleukin (IL)-33 in hepatocellular carcinoma (HCC). Methods: Using immunohisto - chemistry, this prospective study compared IL-33 protein levels in samples of HCC tissue and normal tissue adjacent to the tumour in 60 patients with HCC, and in normal liver tissue from six healthy controls. Interferon (IFN)-α, IFN-γ and IL-33 serum levels were also analysed by enzyme-linked immunosorbent assay in HCC 30 patients and 10 healthy controls. The level of IL-33 immunohistochemical staining was compared with the rate of lymph node metastasis in HCC patients. Results: IL-33 was strongly positive in the cytoplasm of hepatocytes. The median percentage of IL-33-positive tissue was higher in HCC than in normal liver tissue samples (adjacent to the tumour or fromcontrols). Serum IFN-α, IFN-γ and IL-33 levels were higher in pre- and postoperative samples from HCC patients than in control samples, and in patients with metastasis compared with those without metastasis. Conclusions: Increased IL-33 protein levels were observed in serum and liver tissue from HCC patients; IL-33 may be a useful biological marker for monitoring HCC growth and metastasis.