Improved Bone Regeneration With Multiporous PLGA Scaffold and BMP-2-Transduced Human Adipose-Derived Stem Cells by Cell-Permeable Peptide
Improved Bone Regeneration With Multiporous PLGA Scaffold and BMP-2-Transduced Human Adipose-Derived Stem Cells by Cell-Permeable Peptide
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DOI：
10.1097/id.0000000000000523
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发表时间：
2017-02-01
影响因子：
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通讯作者：
Pyo, Sung-woon
中科院分区：
文献类型：
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作者：
Park, Suhyun;Heo, Hyun-A;Pyo, Sung-woon
目的：目前，许多工作集中在利用脂肪源性干细胞（ADSC）进行骨工程，其分化为成骨细胞。本研究旨在探讨基因修饰的脂肪干细胞的骨再生能力。材料与方法：体外培养脂肪干细胞，用重组腺病毒转染表达骨形态发生蛋白-2（rAd/BMP-2）。在24只大鼠的顶骨上创建两个5 mm的全层骨缺损。将缺损空着（n = 12），单独用支架修复（n = 12），用成骨培养基中的ADSC移植（n = 12），或用rAd/BMP-2转导的ADSC移植（n = 12）。每组6个缺陷进行了评估，通过组织学观察，组织形态计量学分析，和显微计算机断层扫描（micro-CT）imaging在4和8 weeks aftertransplantation.Results：增加新骨形成观察rAd/BMP-2-transduced ADSC组，与其他组相比。micro-CT显示，rAd/BMP-2转染的ADSCs组在两个时间点的骨体积/组织体积比与其他组相比差异有统计学意义（P < 0.05）。结论：转染BMP-2可促进ADSCs的成骨分化，促进骨再生。在本研究的局限性下，BMP-2基因修饰的脂肪干细胞有望应用于临床。
Objective: Currently, much work has focused on the engineering of bone using adipose-derived stem cells (ADSCs), which differentiate into osteogenic cells. This study was conducted to assess the bone-regenerating capacity of ADSCs with genetic modification.Materials and Methods: ADSCs were cultured and transduced with recombinant adenovirus-expressing bone morphogenetic protein-2 (rAd/BMP-2). Two 5-mm full-thickness bone defects were created on the parietal bones of 24 rats. The defects were left empty (n = 12), restored with a scaffold alone (n = 12), transplanted with ADSCs in osteogenic media (n = 12), or transplanted with rAd/BMP-2-transduced ADSCs (n = 12). Six defects from each group were assessed by histologic observation, histomorphometric analysis, and microcomputed tomography (micro-CT) imaging at 4 and 8 weeks after transplantation.Results: Increased new bone formation was observed in the rAd/BMP-2-transduced ADSC groups, compared with the other groups. On micro-CT, significant differences were noted in bone volume-totissue volume ratios between rAd/BMP-2-transduced ADSCs group and the other groups at both time points (P < 0.05).Conclusion: The result demonstrates that transferring BMP-2 promotes the osteogenic differentiation of ADSCs and enhances bone regeneration. Under limitation of this study, genetic modification of ADSCs with BMP-2 could be adopted in clinical application.