A perivascular origin for mesenchymal stem cells in multiple human organs
A perivascular origin for mesenchymal stem cells in multiple human organs
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DOI：
10.1016/j.stem.2008.07.003
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发表时间：
2008-09-11
期刊：
影响因子：
23.9
通讯作者：
Peault, Bruno
中科院分区：
文献类型：
--
作者：
Crisan, Mihaela;Yap, Solomon;Peault, Bruno
间充质干细胞（MSCs）是来源于发达器官培养的典型的多能祖细胞，其身份和天然分布尚不清楚。我们前瞻性地鉴定了包括骨骼肌、胰腺、脂肪组织和胎盘在内的多种人体器官中的血管周围细胞，主要是周细胞，CD146、NG2和PDGF-R β表达和造血、内皮和肌源性细胞标志物的缺失。从骨骼肌或非肌肉组织中纯化的血管周围细胞在培养和体内都是肌源性的。无论其组织来源如何，长期培养的血管周围细胞保留了肌原性；在克隆水平上表现出成骨、成软骨和成脂肪的潜能；表达MSC标记物；并在趋化性的培养模式下迁移。在天然和非培养的血管周围细胞表面也检测到MSC标志物的表达。因此，血管壁有一个祖细胞储备，这可能是难以捉摸的间充质干细胞和其他相关成体干细胞起源的组成部分。
Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-R beta expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or nonmuscle tissues were myogenic in culture and in vivo. Irrespective of their tissue origin, long-term cultured perivascular cells retained myogenicity; exhibited at the clonal level osteogenic, chondrogenic, and adipogenic potentials; expressed MSC markers; and migrated in a culture model of chemotaxis. Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells.