Assessment of blood lactate: practical evaluation of the Biosen 5030 lactate analyzer.
Assessment of blood lactate: practical evaluation of the Biosen 5030 lactate analyzer.
复制标题
血乳酸评估：Biosen 5030 乳酸分析仪的实际评估。
DOI：
10.1097/00005768-200001000-00036
复制
发表时间：
2000
影响因子：
4.1
通讯作者：
S. Bird
中科院分区：
文献类型：
--
作者：
R. Davison;D. Coleman;J. Balmer;M. Nunn;S. Theakston;M. Burrows;S. Bird
目的
本研究的目的是在实际实验室研究背景下，评估Biosen 5030乳酸分析仪与YSI 2300乳酸分析仪和Kodak Ektachem DTII相比的有效性和可靠性。
方法
为了评估有效性，使用三台分析仪分析了144份一式三份的毛细血管化血液样本，这些样本的值在一定范围内。为了评估可靠性，对另外665份样本进行了重复分析。通过在室温下储存7至20 h后重新分析静息和最大运动血样，确定时间稳定性。为了测量研究者间和研究者内的可靠性，由不同的研究者从三个不同的受试者中采集20个静息样本，并确定变异系数。
结果
Biosen、YSI（r2 = 0.97）和Kodak Ektachem（r2 = 0.91）之间存在强相关性。Biosen与YSI相比的分析显示正偏倚为0.37 mmol x L（-1）（95%一致性限度，-0.85至1.59 mmol x L（-1））。重测信度相关显著（r2 = 0.99，P < 0.05），配对t检验显示差异较小（0.03 mmol × L（-1），P < 0.05）。三名研究者在20份样本中的变异系数范围为1.3%至3%。在暴露于空气中7小时后，静息血液样本中的血乳酸浓度显著增加（0.2 mmol x L（-1），P < 0.05），而暴露于空气中20小时后，最大运动血乳酸值没有变化。
结论
在实际应用中，Biosen 5030乳酸盐分析仪与其他分析仪相当，可在整个数值范围内快速可靠地测量血液乳酸盐浓度，并在室温下长时间保持稳定。
PURPOSE
The aim of this study was to assess the validity and reliability of the Biosen 5030 lactate analyzer compared with a YSI 2300 lactate analyzer and a Kodak Ektachem DTII in a practical laboratory study context.
METHODS
To assess validity, 144 triplicate capillarized blood samples, across a range of values, were analyzed using the three analyzers. To assess reliability a further 665 samples were repeat analyzed. Temporal stability was determined by the reanalysis of resting and maximal exercise blood samples, after a period of storage ranging from 7 to 20 h, at room temperature. To measure inter- and intra-investigator reliability, 20 resting samples were taken from three different subjects by different investigators and a coefficient of variation was determined.
RESULTS
There were strong relationships between the Biosen, the YSI (r2 = 0.97), and the Kodak Ektachem (r2 = 0.91). An analysis of Biosen compared with YSI revealed a positive bias of 0.37 mmol x L(-1) (95% limits of agreement, -0.85 to 1.59 mmol x L(-1)). The test-retest reliability correlation was significant (r2 = 0.99, P < 0.05), but a paired t-test revealed a small (0.03 mmol x L(-1), P < 0.05) significant difference. The coefficient of variation from the three investigators across the 20 samples ranged from 1.3 to 3%. Blood lactate concentration in resting blood samples did significantly increase in value (0.2 mmol x L(-1), P < 0.05) after 7-h exposure to the air, whereas there was no change in maximal exercise blood lactate values after 20-h exposure to the air.
CONCLUSIONS
In a practical context, the Biosen 5030 lactate analyzer was comparable to the other analyzers giving fast reliable measures of blood lactate concentrations over the full range of values, which remained stable over extended periods at room temperature.