Battery-triggered ultrasensitive electrochemiluminescence detection on microfluidic paper-based immunodevice based on dual-signal amplification strategy
Battery-triggered ultrasensitive electrochemiluminescence detection on microfluidic paper-based immunodevice based on dual-signal amplification strategy
复制标题
基于双信号放大策略的微流控纸基免疫装置电池触发超灵敏电化学发光检测
DOI：
10.1016/j.aca.2012.12.053
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发表时间：
2013-03-12
影响因子：
6.2
通讯作者：
Yu, Jinghua
中科院分区：
文献类型：
--
作者：
Li, Weiping;Li, Meng;Yu, Jinghua
研究了在微流控纸基分析装置（mu-PADs）上进行超灵敏电化学发光（ECL）多路免疫分析的双信号放大策略。采用氧化石墨烯-壳聚糖/金纳米颗粒（GCA）免疫传感平台和[4,4'-（2,5-二甲氧基-1,4-苯基）双（乙炔-2,1-二酰基）二苯甲酸]（p -酸）功能化纳米孔银（p -酸/NPS）信号放大标记实现了双信号放大技术。为了进一步的低成本和一次性应用，在自制的电压可调电源装置的帮助下，将电池触发的恒电位ECL （p -酸标签+1.0V（相对于Ag/AgCl辅助电极））应用于基于纸张的免疫装置，从而放弃了传统的电化学工作站。我们发现两种肿瘤标志物可以在0.003-20和0.001-10 ngmL（-1）的线性范围内依次检测到，检测限分别降至1.0和0.8 pgmL（-1）。结果表明，该免疫检测方法具有良好的精密度和高灵敏度，同时也证明了该电池触发ECL纸基免疫装置可提供快速、简单、同时进行的多重免疫检测，具有高通量、低成本和低检出限的特点。(C) 2013 Elsevier B.V.版权所有
Dual-signal amplification strategy for ultrasensitive electrochemiluminescence (ECL) multiplexed immunoassay on microfluidic paper-based analytical devices (mu-PADs) was demonstrated. This dual-signal amplification technique was achieved by employing graphene oxide-chitosan/gold nanoparticles (GCA) immunosensing platform and [4,4'-(2,5-dimethoxy-1,4-phenylene)bis(ethyne-2,1-diyl) dibenzoic acid] (P-acid) functionalized nanoporous silver (P-acid/NPS) signal amplification label. For further low-cost and disposable applications, battery-triggered constant-potential ECL (+1.0V for P-acid label (vs. Ag/AgCl auxiliary electrode)) was applied on this paper-based immunodevice with the aid of a home-made voltage-tunable power device, allowing the traditional electrochemical workstation to be abandoned. We found that two tumor markers could be sequentially detected in the linear ranges of 0.003-20 and 0.001-10 ngmL(-1) with the detection limits down to 1.0 and 0.8 pgmL(-1), respectively, by simply reversing the connection mode on two working electrodes. The results exhibited excellent precision and high sensitivity of such immunoassay, and it also demonstrated that this battery-triggered ECL paper-based immunodevice could provide a rapid, simple and simultaneous multiplex immunoassay with high throughput, low-cost and low detection limits for point-of-care testing. (C) 2013 Elsevier B.V. All rights reserved.