Targeting TRPV1 for Body Weight Control using TRPV1(-/-) Mice and Electroacupuncture.
Targeting TRPV1 for Body Weight Control using TRPV1(-/-) Mice and Electroacupuncture.
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DOI：
10.1038/srep17366
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发表时间：
2015-12-01
影响因子：
4.6
通讯作者：
Lin YW
中科院分区：
文献类型：
--
作者：
Choowanthanapakorn M;Lu KW;Yang J;Hsieh CL;Lin YW
肥胖是一个全球性的社会医学问题，其发病率高达20 - 30%。本研究通过电针来观察TRPV1的调控是否能控制小鼠体重。结果表明，对照组体重随时间增加（108.19 ± 1.31%，n = 7）。电针组小鼠体重增加明显低于对照组（104.41 ± 0.76%，p <0.05，n = 7），而假电针组小鼠体重增加不明显（109.1 ± 0.63%，p <0.05，n = 7）。电针并没有降低TRPV1基因敲除小鼠的体重增长（TRPV1-/-和TRPV1-/-+电针分别为107.94 ± 0.41%和107.79 ± 1.04%，p> 0.05）。电针组内脏白色脂肪组织（WAT）重量在4周后较低。此外，在电针治疗后，背根神经节（DRG）和脊髓（SC）中TRPV1，pPKA，pPKC和pERK的蛋白水平增加，但在假电针和TRPV1 −/−小鼠中没有增加。这项研究表明，靶向TRPV1有利于控制小鼠的体重和TRPV1相关机制。
Obesity is a global social medical problem resulting in morbidity as high as 20–30%. Here we investigated whether the manipulation of TRPV1 can control mice body weight through electroacupuncture (EA). The results demonstrated that body weight increased with time in the control group (108.19 ± 1.31%, n = 7). The increase of mice body weight was significantly less in the EA group (104.41 ± 0.76%, p < 0.05, compared with the control group, n = 7) but not in the sham EA group (109.1 ± 0.63%, p < 0.05, compared with EA group, n = 7). EA did not decrease the gain of body weight in TRPV1 knock mice (107.94 ± 0.41% and 107.79 ± 1.04% for TRPV1−/− and TRPV1−/− with EA, respectively, p > 0.05). The visceral white adipose tissue (WAT) weight was lower in the EA group at 4 weeks after manipulation. Moreover, the protein levels of TRPV1, pPKA, pPKC, and pERK were increased in the dorsal root ganglion (DRG) and spinal cord (SC) after EA treatment but not in the sham EA and TRPV1−/− mice. This study suggests that targeting TRPV1 is beneficial in controlling body weight and TRPV1-associated mechanisms in mice.