HY5 Interacts with the Histone Deacetylase HDA15 to Repress Hypocotyl Cell Elongation in Photomorphogenesis
HY5 Interacts with the Histone Deacetylase HDA15 to Repress Hypocotyl Cell Elongation in Photomorphogenesis
复制标题
HY5 与组蛋白脱乙酰酶 HDA15 相互作用抑制光形态发生中的下胚轴细胞伸长
DOI：
10.1104/pp.19.00055
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发表时间：
2019
期刊：
影响因子：
7.4
通讯作者：
Liu Xuncheng
中科院分区：
文献类型：
--
作者：
Zhao Linmao;Peng Tao;Chen Chia-Yang;Ji Rujun;Gu Dachuan;Li Tingting;Zhang Dongdong;Tu Yi-Tsung;Wu Keqiang;Liu Xuncheng
光形态发生是一个重要的植物发育过程，涉及光介导的转录组和组蛋白修饰变化。转录因子伸长下cotyl5（伸长下cotyl5， HY5）作用于光感受器多个家族的下游，通过调节光响应基因的表达来促进光形态发生。然而，hy5介导的转录调控的分子机制仍不清楚。在这里，我们证明HY5在体外和体内都直接与降低钾依赖性3/组蛋白去乙酰化酶1 （HDA1）型组蛋白去乙酰化酶HDA15相互作用。表型分析表明，HDA15在红光和远红光条件下都是拟南芥幼苗下胚轴细胞伸长的负调控因子。抑制下胚轴伸长需要HDA15的酶活性。此外，HDA15和HY5在光形态形成过程中相互依赖地抑制下胚轴细胞的伸长。全基因组转录组分析显示，HDA15和HY5共同抑制细胞壁组织和生长素信号相关基因的转录。此外，在拟南芥幼苗中，HY5抑制下胚轴细胞伸长相关基因的功能需要HDA15。此外，HY5将HDA15招募到靶基因的启动子上，并以光依赖的方式通过降低组蛋白H4乙酰化水平来抑制基因表达。我们的研究揭示了一个关键的转录调控节点，其中HY5与HDA15相互作用，参与抑制下胚轴细胞伸长以促进光形态形成。
Photomorphogenesis is a critical plant developmental process that involves light-mediated transcriptome and histone modification changes. The transcription factor ELONGATED HYPOCOTYL5 (HY5) acts downstream of multiple families of photoreceptors to promote photomorphogenesis by regulating the expression of light-responsive genes. However, the molecular mechanism for HY5-mediated transcriptional regulation remains largely unclear. Here, we demonstrated that HY5 directly interacts with a Reduced Potassium Dependence3/Histone Deacetylase1 (HDA1)-type histone deacetylase, HDA15, both in vitro and in vivo. Phenotypic analysis revealed that HDA15 is a negative regulator of hypocotyl cell elongation under both red and far-red light conditions in Arabidopsis (Arabidopsis thaliana) seedlings. The enzymatic activity of HDA15 is required for inhibition of hypocotyl elongation. Furthermore, HDA15 and HY5 act interdependently in the repression of hypocotyl cell elongation in photomorphogenesis. Genome-wide transcriptome analysis revealed that HDA15 and HY5 corepress the transcription of a subset of cell wall organization and auxin signaling-related genes. In addition, HDA15 is required for the function of HY5 in the repression of genes related to hypocotyl cell elongation in Arabidopsis seedlings. Moreover, HY5 recruits HDA15 to the promoters of target genes and represses gene expression by decreasing the levels of histone H4 acetylation in a light-dependent manner. Our study revealed a key transcription regulatory node in which HY5 interacts with HDA15 involved in repressing hypocotyl cell elongation to promote photomorphogenesis.