Virus-Induced Diabetes Mellitus: Glucose Abnormalities Produced in Mice by the Six Members of the Coxsackie B Virus Group
Virus-Induced Diabetes Mellitus: Glucose Abnormalities Produced in Mice by the Six Members of the Coxsackie B Virus Group
复制标题
病毒引起的糖尿病：柯萨奇 B 病毒组的六种成员导致小鼠血糖异常
DOI：
10.2337/diab.31.6.496
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发表时间：
1982
期刊：
影响因子：
7.7
通讯作者：
A. Notkins
中科院分区：
文献类型：
--
作者：
A. Toniolo;T. Onodera;G. Jordan;J. Yoon;A. Notkins
摘要 在各种细胞类型中传代之前和之后，研究了柯萨奇 B 病毒 (CBV) 在小鼠体内产生糖尿病的能力。在猴肾细胞或小鼠胚胎成纤维细胞中传代的CBV未能产生异常的葡萄糖耐量测试，而病毒在小鼠胰腺或β细胞培养物中传代五次或更多次则产生短暂的异常葡萄糖耐量测试。免疫荧光和组织学研究表明，CBV 在培养的 β 细胞中传代改变了这些病毒的趋向性，从腺泡胰腺转移到朗格汉斯岛。尽管在 β 细胞培养物中传代的所有六种 CBV 血清型的表现非常相似，但在不同的病毒传代中观察到了显着的差异，并且在一些实验中，β 细胞损伤和葡萄糖异常是最小的。从这些和其他实验中，我们得出结论，CBV 组的六名成员有可能感染和破坏小鼠的胰腺 β 细胞。
SUMMARY The capacity of Coxsackie B viruses (CBVs) to produce diabetes in mice was studied before and after passage in various cell types. CBVs that had been passaged in monkey kidney cells or in mouse embryo fibroblasts failed to produce abnormal glucose tolerance tests, whereas virus passaged five or more times in the pancreata of mice or in beta-cell cultures p oduced transient abnormal glucose tolerance tests. Immunofluorescence and histologic studies revealed that passage of CBVs in cultured beta-cells changed the tropism of these viruses from the acinar pancreas to the islets of Langerhans. Although all six CBV serotypes that had been passaged in beta-cell cultures behaved very similarly, substantial variation was observed with the different virus passages and in some experiments, beta-cell damage and the glucose abnormalities were minimal. From these and other experiments, we conclude that the six members of the CBV group have the potential for infecting and damaging pancreatic beta-cells in mice.