Three phases of phagocyte contribution to resistance against Listeria monocytogenes.
Three phases of phagocyte contribution to resistance against Listeria monocytogenes.
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吞噬细胞对单核细胞增生李斯特菌抵抗力的三个阶段。
DOI：
10.1099/00221287-106-1-165
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
S. Shimotori
中科院分区：
文献类型：
--
作者：
M. Mitsuyama;K. Takeya;K. Nomoto;S. Shimotori
在远交 ddN 小鼠中分析了吞噬细胞对单核细胞增生李斯特菌的保护作用。以3×103至4×103剂量静脉注射的大部分细菌在10分钟内被截留在肝脏中。 6 小时内细菌数量短暂减少了 10 倍。初始阶段的抗李斯特菌活性对X射线有抵抗力，但被卡拉胶抑制，且不受免疫影响。感染早期阶段的保护似乎归因于固定巨噬细胞的功能。器官中的活细菌从 6 小时到 72 小时逐渐但缓慢地增加，达到最大数量。 X射线照射或角叉菜胶处理显着增强了这一时期的细菌生长。游离吞噬细胞的积累似乎抑制了这一阶段的细菌生长。细菌数量从第 4 天开始减少，到第 9 天变得无法检测到。最后阶段对细菌生长的抑制作用可能依赖于免疫激活的巨噬细胞，并可通过 X 射线辐射和角叉菜胶逆转。局部感染的病程与全身感染相似，只是最初没有下降。我们的结论是，根据吞噬细胞在抵抗中的作用，单核细胞增生李斯特菌的感染过程可分为三个阶段。
The contribution of phagocytes to protection against Listeria monocytogenes was analysed in outbred ddN mice. Most of the bacteria injected intravenously at a dose of 3 × 103 to 4 × 103 were trapped in the liver within 10 min. There was a transient 10-fold decrease in the number of bacteria by 6 h. Anti-listeria activity in the initial phase was resistant to X-irradiation but was inhibited by carrageenan, and was not influenced by immunization. The protection in this very early stage of infection seemed to be attributable to the function of fixed macrophages. Viable bacteria in the organs increased progressively but slowly from 6 h to 72 h to reach maximum numbers. Bacterial growth during this period was markedly enhanced by X-irradiation or treatment with carrageenan. Accumulation of free phagocytes seemed to suppress the bacterial growth in this phase. The number of bacteria began to decrease from day 4 and became undetectable by day 9. The suppressive effect on bacterial growth in this last phase may be dependent on immunologically activated macrophages and was reversed by X-irradiation and carrageenan. The course of local infection was similar to that of systemic infection except for the lack of initial decrease. We conclude that the course of infection with L. monocytogenes can be divided into three phases with regard to the roles of phagocytes in resistance.