Small molecule analysis and imaging of fatty acids in the zebra finch song system using time-of-flight-secondary ion mass spectrometry.
Small molecule analysis and imaging of fatty acids in the zebra finch song system using time-of-flight-secondary ion mass spectrometry.
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DOI：
10.1111/j.1471-4159.2011.07274.x
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发表时间：
2011-08
影响因子：
4.7
通讯作者：
Clayton DF
中科院分区：
文献类型：
--
作者：
Amaya KR;Sweedler JV;Clayton DF
脂肪酸是大脑新陈代谢和信号传递的中心，但它们在复杂的大脑回路中的分布一直很难研究。在这里，我们应用了一种新兴的技术，飞行时间二次离子质谱仪(ToF-SIMS)，在一个良好的大脑回路化学分析模型系统中对特定的脂肪酸进行成像，斑纹草雀(Taeniopygia Guttata)。斑马雀是一种鸣禽，在一组相互连接的离散的、专门的大脑核团“歌核”的控制下，发出复杂的学习发声。利用ToF-SIMS，主要的歌声核团通过其必需和非必需脂肪酸含量的差异而被可视化。必需脂肪酸(花生四烯酸和二十二碳六烯酸)在宋氏核团中有不同的分布，18碳脂肪酸硬脂酸和油酸区分了宋氏核心区和壳核区。光谱数据集的主成分分析提供了宋核之间化学差异的进一步证据。通过对青少年歌曲学习过程中三个不同年龄段的歌曲核团RA的分析，我们首次获得了与歌曲学习进程相关的脂质含量变化的直接证据。结果表明，ToF-SIMS为研究脂质在脑组织、发育和功能中的作用提供了一个良好的模型系统。
Fatty acids are central to brain metabolism and signaling, but their distributions within complex brain circuits have been difficult to study. Here we applied an emerging technique, time-of-flight secondary ion mass spectrometry (ToF-SIMS), to image specific fatty acids in a favorable model system for chemical analyses of brain circuits, the zebra finch (Taeniopygia guttata). The zebra finch, a songbird, produces complex learned vocalizations under the control of an interconnected set of discrete, dedicated brain nuclei “song nuclei”. Using ToF-SIMS, the major song nuclei were visualized by virtue of differences in their content of essential and non-essential fatty acids. Essential fatty acids (arachidonic acid and docosahexaenoic acid) showed distinctive distributions across the song nuclei, and the 18-carbon fatty acids stearate and oleate discriminated the different core and shell subregions of song nucleus LMAN. Principle component analysis of the spectral data set provided further evidence of chemical distinctions between the song nuclei. By analyzing song nucleus RA at three different ages during juvenile song learning, we obtain the first direct evidence of changes in lipid content that correlate with progression of song learning. The results demonstrate the value of ToF-SIMS to study lipids in a favorable model system for probing the function of lipids in brain organization, development and function.