GELATION OF SICKLE-CELL HEMOGLOBIN .2. METHEMOGLOBIN
GELATION OF SICKLE-CELL HEMOGLOBIN .2. METHEMOGLOBIN
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DOI：
10.1016/0022-2836(74)90050-3
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发表时间：
1974-01-01
影响因子：
5.6
通讯作者：
EWERT, SM
中科院分区：
文献类型：
--
作者：
BRIEHL, RW;EWERT, SM
通过先前描述的平衡超离心法测定镰状细胞高铁血红蛋白的凝胶形成。取决于条件，在0.35至0.5 g/ml之间的浓度下，发生从溶胶到凝胶的相变，表明凝胶化，远高于之前观察到的脱氧血红蛋白S凝胶化的浓度。肌醇六磷酸有利于凝胶化，但在不存在肌醇六磷酸的情况下也观察到凝胶化。将pH降低至6有利于胶凝。如果假设凝胶化需要T四级构象的分子，这些结果提供了进一步的证据，高铁血红蛋白存在于溶液中的R-T平衡，并且这种平衡位于脱氧血红蛋白（T-状态）和一氧化碳或氧合血红蛋白（R-状态）所表现出的极端之间。
Sickle cell methaemoglobin was assayed for gel formation by an equilibrium ultracentrifugation method previously described. A phase change from sol to gel, indicative of gelation, occurred, depending on conditions, at concentrations between 0.35 and 0.5 g/ml, considerably higher than concentrations observed previously for gelation of deoxyhaemoglobin S. Inositol hexaphosphate favours gelation, but gelation is seen also in its absence. Lowering pH toward 6 favours gelation. If gelation is assumed to require molecules in the T quaternary conformation, these results provide further evidence that methaemoglobin exists in R-T equilibrium in solution and that this equilibrium lies between the extremes exhibited by deoxyhaemoglobin (T-state) and carbon monoxide or oxyhaemoglobin (R-state).