The effect of single and repeated bouts of prolonged cycling and circadian variation on saliva flow rate, immunoglobulin A and α-amylase responses
The effect of single and repeated bouts of prolonged cycling and circadian variation on saliva flow rate, immunoglobulin A and α-amylase responses
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DOI：
10.1080/02640410410001716733
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发表时间：
2004-01-01
影响因子：
3.4
通讯作者：
Gleeson, M
中科院分区：
文献类型：
--
作者：
Li, TL;Gleeson, M
本研究的目的是确定不同时间段的运动对唾液流率、免疫球蛋白A(SIgA)浓度和分泌率以及α-淀粉酶活性的影响，以及在同一天进行第二次运动后这些参数是如何变化的。在平衡设计中，八名男性志愿者参加了三个相隔至少4天的实验试验。在只进行下午锻炼的试验中，参与者从14：00时开始以60%最大摄氧量骑自行车2小时。在另外两个试验中，参与者进行两次60%最大摄氧量2小时的锻炼(第一次开始于09：00小时，第二次开始于14：00小时)或单独进行一次静息试验。分别于运动前10min、运动后58~60min、运动后最后2min、运动后1h、2 h采集唾液样本。分别于运动前5min和运动后即刻采静脉血。受试者在试验前一天的23：00到试验当天的18：00之间保持禁食。唾液α-淀粉酶分泌率从早晨的最低值到傍晚的最高值，随着时间的推移而增加。60%VO2max循环2 h可显著降低唾液流率，升高SIgA浓度和α-淀粉酶活性，但对SIgA分泌率无明显影响。在一天中的不同时间进行长时间的骑行在短期内不会对唾液和血浆激素反应产生不同的影响。进行第二轮长时间的锻炼会引起更大的血浆应激激素反应，但似乎并不会严重影响口服免疫力。这些发现还表明，就唾液分泌、SIgA和α-淀粉酶反应而言，休息3小时足以从先前的剧烈运动中恢复过来。在这种运动中，交感神经刺激似乎足够强，足以抑制唾液流率；然而，它似乎不会通过跨细胞作用增加SIgA的产量。
The purpose of this study was to establish the effect of exercise at different times of day on saliva flow rate, immunoglobulin A (sIgA) concentration and secretion rate, and alpha-amylase activity, and to establish how these parameters change following a second exercise bout performed on the same day. In a counterbalanced design, eight male volunteers participated in three experimental trials separated by at least 4 days. On the trial with afternoon exercise only, the participants cycled for 2 h at 60% VO2max starting at 14:00 h. On the other two trials, participants performed either two bouts of exercise at 60% VO2max for 2 h (the first started at 09:00 h and the second started at 14:00 h) or a separate resting trial. Unstimulated saliva samples were obtained 10 min before exercise, after 58 - 60 min and during the last 2 min of exercise, and at 1 h and 2 h after exercise. Venous blood samples were taken 5 min before exercise and immediately after exercise for both bouts. Participants remained fasted between 23:00 h on the day before the trials and 18:00 h on the day of the trial. Circadian variations were found in sIgA concentration, which decreased with time from its highest value in the early morning to its lowest value in the evening, and salivary alpha-amylase secretion rate, which increased from its lowest value in the morning to its highest value in the late afternoon. Cycling at 60% VO2max for 2 h significantly decreased saliva flow rate, increased sIgA concentration and alpha-amylase activity, but did not influence sIgA secretion rate. Performing prolonged cycling at different times of day did not differentially affect the salivary and plasma hormonal responses in the short term. Performance of a second prolonged exercise bout elicited a greater plasma stress hormone response but did not appear to compromise oral immunity acutely. These findings also suggest that, in terms of saliva secretion, sIgA and alpha-amylase responses, a 3 h rest is enough to recover from previous strenuous exercise. During such exercise, sympathetic stimulation appears to be strong enough to inhibit saliva flow rate; however, it appears that it does not increase sIgA output via transcytosis.