Clinicopathological significance of p16, cyclin D1, Rb and MIB-1 levels in skull base chordoma and chondrosarcoma.
Clinicopathological significance of p16, cyclin D1, Rb and MIB-1 levels in skull base chordoma and chondrosarcoma.
复制标题
DOI：
10.1016/j.wjorl.2015.09.005
复制
发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
Wang ZL
中科院分区：
文献类型：
--
作者：
Liu JQ;Zhang QH;Wang ZL
探讨p16、cyclin D1、Rb、MIB-1在颅底脉络膜和软骨肉瘤组织中的表达及其临床病理意义。对100例颅底脉络膜炎、30例软骨肉瘤和20例正常软骨组织进行免疫组化分析。p16、cyclinD 1、Rb和MIB-1蛋白的表达水平与临床病理特征的潜在相关性进行了评估。与正常软骨标本（对照）相比，在颅底脉络膜和软骨肉瘤标本中，p16蛋白表达降低，cyclin D1、Rb和MIB-1蛋白表达升高。在p16阴性、cyclin D1和Rb阳性的颅底脉络膜标本中，MIB-1 LI水平明显增高（P < 0.05）。颅底软骨肉瘤组织中MIB-1 LI明显增高，而p16、cyclin D1和Rb均为阴性表达（P < 0.05）。在颅底脉络膜中，p16与cyclin D1、Rb呈负相关，而cyclin D1与Rb呈正相关。p16、cyclin D1、Rb和MIB-1的表达与颅底脉络膜炎患者的年龄、性别和病理类型无关（P > 0.05）。p16、Rb和MIB-1的表达与肿瘤的数目有关（P < 0.05）。此外，p16和MIB-1的表达似乎与颅底脉络膜炎患者的预后相关。p16、cyclin D1和Rb蛋白的异常表达可能与颅底脉络膜和软骨肉瘤的发生有关。
To investigate the expression of p16, cyclin D1, retinoblastoma tumor suppressor protein (Rb) and MIB-1 in skull base chordoma and chondrosarcoma tissues, and to determine the clinicopathological significance of the above indexes in these diseases. A total of 100 skull base chordoma, 30 chondrosarcoma, and 20 normal cartilage tissue samples were analyzed by immunohistochemistry. The expression levels of p16, cyclinD1, Rb and MIB-1 proteins were assessed for potential correlation with the clinicopathological features. As compared to normal cartilage specimen (control), there was decreased expression of p16, and increased expression of cyclin D1, Rb and MIB-1 proteins, in both skull base chordoma and chondrosarcoma specimens. MIB-1 LI levels were significantly increased in skull base chordoma specimens with negative expression of p16, and positive expression of cyclin D1 and Rb (P < 0.05). Significantly elevated MIB-1 LI was also detected in skull base chondrosarcoma tissues, while there was negative expression of p16, cyclin D1 and Rb (P < 0.05). In skull base chordoma, p16 negatively correlated with cyclin D1 and Rb, while cyclin D1 positively correlated with Rb. Additionally, p16, cyclin D1, Rb, or MIB-1 expression showed no correlation with age, gender, or pathological classification of patients with skull base chordoma (P > 0.05). However, p16 and MIB-1 levels correlated with the intradural invasion, and expression of p16, Rb and MIB-1 correlated with the number of tumor foci (P < 0.05). Further, the expression of p16 and MIB-1 appeared to correlate with the prognosis of patients with skull base chordoma. The abnormal expression of p16, cyclin D1 and Rb proteins might be associated with the tumorigenesis of skull base chordoma and chondrosarcoma.