Proadrenomedullin N-terminal 20 peptide (PAMP) immunoreactivity in vertebrate juxtaglomerular granular cells identified by both light and electron microscopy
Proadrenomedullin N-terminal 20 peptide (PAMP) immunoreactivity in vertebrate juxtaglomerular granular cells identified by both light and electron microscopy
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DOI：
10.1006/gcen.1999.7365
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发表时间：
1999-11-01
影响因子：
2.7
通讯作者：
Cuttitta, F
中科院分区：
文献类型：
--
作者：
López, J;Cuesta, N;Cuttitta, F
肾上腺髓质素（AM）基因是一种多功能肽类激素，在哺乳动物肾组织中表达，并已显示刺激肾素释放。这种肽及其基因相关的伙伴，肾上腺髓质素前体N-末端20肽（PAMP），在肾脏中的确切细胞来源仍然不确定。在本研究中，我们已经确定了PAMP免疫反应细胞在不同的哺乳动物物种，包括人，通过光学显微镜。此外，这些细胞已在小鼠肾脏中通过光镜和电镜技术进行了进一步研究。在光镜水平上，PAMP免疫标记优先位于扩大的肾小球传入小动脉的内皮下细胞中，即在肾小球细胞中。然而，这些细胞不显示AM的免疫标记。在电镜水平，免疫染色出现在肾小球细胞的含肾素的分泌颗粒内。这些结果证实了肾素和AM肽家族之间的直接联系，并为研究AM和PAMP在控制肾素活性中的潜在调节功能提供了形态学基础。相比之下，既不AM也不PAMP免疫反应性检测在非哺乳动物脊椎动物的肾脏，比在特定物种的血管，提供了一个新的系统发育差异，哺乳动物和非哺乳动物脊椎动物之间的肾小球器官。(C)北京：科学出版社.
The gene for adrenomedullin (AM), a multifunctional peptide hormone, is expressed in mammalian renal tissue and has been shown to stimulate renin release. The exact cell source of this peptide and its gene-related partner, proadrenomedullin N-terminal 20 peptide (PAMP), in kidney is still uncertain. In the present study we have identified PAMP-immunoreactive cells in the kidney of different mammalian species, including man, by light microscopy. In addition, these cells have been further studied in mouse kidney by both light and electron microscopic techniques. At the light microscopic level, PAMP immunolabeling is preferentially located in the subendothelial cells of the enlarged glomerular afferent arterioles, that is, in the juxtaglomerular cells. However, these cells do not show immunolabeling for AM. At the electron microscopic level, the immunostaining appears inside the renin-containing secretory granules of the juxtaglomerular cells. These results confirm the direct link between renin and the AM peptide family and provide a morphological basis for studying the potential modulatory function of AM and PAMP in the control of renin activity. In contrast, neither AM nor PAMP immunoreactivities were detected in the kidney of nonmammalian vertebrates, other than in blood vessels of particular species, providing a new phylogenetic difference in the juxtaglomerular apparatus between mammalian and nonmammalian vertebrates. (C) 1999 Academic Press.