Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase
Aspergillus fumigatus calcineurin interacts with a nucleoside diphosphate kinase
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DOI：
10.1016/j.micinf.2012.05.003
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发表时间：
2012-09-01
影响因子：
5.8
通讯作者：
Goldman, Gustavo Henrique
中科院分区：
文献类型：
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作者：
Dinamarco, Taisa Magnani;Brown, Neil Andrew;Goldman, Gustavo Henrique
Ca2+-钙调磷酸酶途径影响丝状真菌的毒力和形态发生。在这里，我们鉴定了 37 个与烟曲霉中钙调神经磷酸酶 (CalA) 催化亚基相互作用的 CalA 相互作用蛋白，包括核苷二磷酸激酶 (SwoH)。 CalA 和 SwoH 之间的体内相互作用通过双分子荧光互补进行了验证。 A. fumigatus swoH 是一个必需基因。因此，构建了活性位点 SwoH(V83F) 点突变的温度敏感条件突变菌株，该菌株表现出生长减少和对高温的敏感性增加。 SwoH(V83F)突变并未导致大蜡螟感染模型中毒力的丧失。综上所述，这些结果表明 CalA 与 SwoH 相互作用。 (C) 2012 年巴斯德研究所。由 Elsevier Masson SAS 出版。版权所有。
The Ca2+-calcineurin pathway affects virulence and morphogenesis in filamentous fungi. Here, we identified 37 CalA-interacting proteins that interact with the catalytic subunit of calcineurin (CalA) in Aspergillus fumigatus, including the nucleoside diphosphate kinase (SwoH). The in vivo interaction between CalA and SwoH was validated by bimolecular fluorescence complementation. A. fumigatus swoH is an essential gene. Therefore, a temperature-sensitive conditional mutant strain with a point mutation in the active site, SwoH(V83F), was constructed, which demonstrated reduced growth and increased sensitivity to elevated temperatures. The SwoH(V83F) mutation did not cause a loss in virulence in the Galleria mellonella infection model. Taken together these results imply that CalA interacts with SwoH. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.